Fluorescent imaging to provide visualized evidences for mercury induced hypoxia stress

doi:10.1016/j.jhazmat.2022.130374

YIC-IR

	Fluorescent imaging to provide visualized evidences for mercury induced hypoxia stress
	Zhang, Li 1,4; Zhang, Liangwei1,3 ; Zhang, Xia 1,4,5; Liu, Ping 1,3; Wang, Yue 1,4; Han, Xiaoyue 1,4; Chen, Lingxin1,2,3,5
发表期刊	JOURNAL OF HAZARDOUS MATERIALS
ISSN	0304-3894
	2023-02-15
卷号	444 页码:9
关键词	Hg 2+stress Hypoxia Nitroreductase Fluorescent probe
DOI	10.1016/j.jhazmat.2022.130374
通讯作者	Zhang, Liangwei(liangweizhang@yic.ac.cn) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要	As one typical toxic and dangerous heavy metal, mercury brings incalculable hazards to the environment and human, the mechanism at the molecular level is unclear. There is no visualized evidence to support directly that mercury ions (Hg2+) exposure may induce secondary stress, which is associated with the risk of hypoxia microenvironment in biological systems. Hypoxia occurs in many physiological and pathophysiological processes in the living system, accompanying overexpression of various biomarkers, such as nitroreductase (NTR). Hence, we had successfully developed two NTR-selective fluorescent probes with excellent performance for evaluating the hypoxia degree in vivo and in vitro. We visualized and qualitatively monitored the fluctuations of the endogenous NTR levels in living cells and zebrafish. The imaging results exhibited that different doses of Hg2+ exposure elevated the NTR levels and the same trend in changes of NTR as extrinsic hypoxia exposure, suggesting that Hg2+ exposure induced microenvironmental changes resulting in the hypoxia stress. This is the first time to provide visual evidence to support that Hg2+ stress may involve in the intracellular hypoxia microenvironment through monitoring the dynamic of NTR levels in the living systems. Our results may provide a novel insight into the molecular mechanisms of typical heavy metal element induced toxicity.
资助机构	National Natural Science Foundation of China ; Program of Youth Innovation Promotion Association, CAS ; Taishan Scholar Project Special Funding
收录类别	SCI
语种	英语
关键词[WOS]	OXIDATIVE STRESS ; NITROREDUCTASE DETECTION ; TUMOR-CELLS ; PROBE ; ZEBRAFISH ; EXPOSURE ; STRATEGY ; LEAD
研究领域[WOS]	Engineering ; Environmental Sciences & Ecology
WOS记录号	WOS:000903619500002
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/34815
专题	中国科学院烟台海岸带研究所
通讯作者	Zhang, Liangwei; Chen, Lingxin
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China 2.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Li,Zhang, Liangwei,Zhang, Xia,et al. Fluorescent imaging to provide visualized evidences for mercury induced hypoxia stress[J]. JOURNAL OF HAZARDOUS MATERIALS,2023,444:9.
APA	Zhang, Li.,Zhang, Liangwei.,Zhang, Xia.,Liu, Ping.,Wang, Yue.,...&Chen, Lingxin.(2023).Fluorescent imaging to provide visualized evidences for mercury induced hypoxia stress.JOURNAL OF HAZARDOUS MATERIALS,444,9.
MLA	Zhang, Li,et al."Fluorescent imaging to provide visualized evidences for mercury induced hypoxia stress".JOURNAL OF HAZARDOUS MATERIALS 444(2023):9.