YIC-IR  > 海岸带生物学与生物资源利用重点实验室
FLIM reveals high salinity resistance in a green algae Chlorella sp. under light and dark conditions
Xiao, Zeyu1; Deng, Yifeng1; Sun, Wei1; Wu, Sihan1; Zhang, Zixin2; Wang, Yinchu3,4; Liu, Haipeng1; Zeng, Yijia1; Lu, Zhen1,5; Peng, Xiao1; Wu, Huifeng5; Qu, Junle1
Source PublicationProceedings of SPIE - The International Society for Optical Engineering
ISSN0277786X
2023
Volume12745
KeywordFluorescence imaging Microorganisms
DOI10.1117/12.2683124
AbstractChlorella is a unicellular spherical green microalgae with alternate colors from blue-green to yellowish or red due to different components of innate pigments. Light and salinity are two important environmental factors in Chlorella culture. Light conditions directly affect the growth and biochemical composition of microalgae, while salinity change could influence the pigment composition of Chlorella. Therefore, it has crucial research significance to monitor the response of Chlorella to salinity stress under different light conditions. Recently, fluorescence lifetime imaging microscopy (FLIM) technology has been widely applied into biological fields, providing fluorescence lifetime values for quantitative analysis. Here, FLIM method was used to observe the autofluorescence of a freshwater microalgae, Chlorella sp. Chlorella cells were treated with a series of salinity concentrations (control sample in normal culture medium, 3S sample with an additional 3× salinity, 7S sample with an additional 7× salinity, respectively) under light (12 h/12 h light/dark cycles) or dark (0 h/24 h light/dark cycles) treatments. After one day, images of the microalgae cells from each group were obtained with FLIM system, followed by an analysis with SPCImage software. The results showed that 3× salinity condition had little effect on Chlorella in both light/dark conditions, suggesting the adaptive capacity of Chlorella to seawater salinity. By contrast, the mean fluorescence lifetime values in 7S samples under light conditions were significantly decreased compared to that of the control. Interestingly, similar lifetime values were observed in 7S samples and the control samples under dark conditions, which indicated a potential high salinity resistance induced by different light/dark conditions. In conclusion, FLIM could work as a fast evaluation method of the physiological status of living Chlorella sp. under different culture conditions in a quantitative way. © 2023 SPIE.
Indexed ByEI
Language英语
EI KeywordsFluorescence imaging ; Microorganisms
EI Accession Number20233714731476
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/34293
Collection海岸带生物学与生物资源利用重点实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
Affiliation1.State Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen; 518060, China;
2.School of Pharmacy, Yantai University, Yantai; 264005, China;
3.Key Laboratory of Coastal Biology and Biological Resource Utilization, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Yantai; 264003, China;
4.National Basic Science Data Center, Beijing; 100190, China;
5.CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Yantai Institute of Coastal Zone Research (YIC), Chinese Academy of Sciences (CAS), Shandong Key Laboratory of Coastal Environmental Processes, YICCAS, Yantai; 264003, China
Recommended Citation
GB/T 7714
Xiao, Zeyu,Deng, Yifeng,Sun, Wei,et al. FLIM reveals high salinity resistance in a green algae Chlorella sp. under light and dark conditions[J]. Proceedings of SPIE - The International Society for Optical Engineering,2023,12745.
APA Xiao, Zeyu.,Deng, Yifeng.,Sun, Wei.,Wu, Sihan.,Zhang, Zixin.,...&Qu, Junle.(2023).FLIM reveals high salinity resistance in a green algae Chlorella sp. under light and dark conditions.Proceedings of SPIE - The International Society for Optical Engineering,12745.
MLA Xiao, Zeyu,et al."FLIM reveals high salinity resistance in a green algae Chlorella sp. under light and dark conditions".Proceedings of SPIE - The International Society for Optical Engineering 12745(2023).
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