The invention relates to a field of insecticide, in particular to an oxygen-rich disesquiterpenes compound derived from endophytic fungi of seaweeds, the the preparation method and application of the compound. As shown in a specific formula (I), the preparation method comprises the steps of inoculating aspergillus ustus cf-42 into a fungus liquid medium for ferment and culture, the oxygen-rich disesquiterpenes compound showed in the formula (I) is obtained after a fermentation product is purified. The acquired oxygen-rich disesquiterpenes compound of the present invention is proved by insecticidal activity experiments to achieve an artemia fatality rate of 87.1% with the concentration of 100 mcg/ml and achieve the artemia fatality rate of 50% with the concentration of 65.4 mcg/ml.
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