The invention relates to the field of algicide, insecticide and fungistat, in particular to a polyoxybiotic alkali compound as a seaweed endophytic fungi source, as well as a preparation method and an application thereof. The specific structure formula of the polyoxybiotic alkali compound is disclosed in a formula (I). The preparation method of the polyoxybiotic alkali compound comprises the following steps of : inoculating Aspergillus oryzae cf-2 into a fungi liquid culture medium to be subjected to fermented culture; and after a fermentation product is purified, obtaining the polyoxybiotic alkali compound disclosed in the formula (I). According to the polyoxybiotic alkali compound obtained by the invention, the half inhibitory concentration of the compound is 13.0 micrograms/ milliliter by an algae activity inhibition experiment, and meanwhile, the compound also has the desinsection and bacteriostasis activity.
; 苗凤萍
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