The invention discloses N-pyridylurea chitosan quaternary ammonium salt and a preparation method and an application thereof. The N-pyridylurea chitosan quaternary ammonium salt takes chitosan, methyl chloroformate, aminopyridine and iodomethane as raw materials, the preparation method comprises the following steps : preparing N-methoxycarbonyl chitosan, reacting N-methoxycarbonyl chitosan and aminopyridine, reacting the obtained N-pyridylurea chitosan and iodomethane, and purifying the product to obtain the N-pyridylurea chitosan quaternary ammonium salt. The prepared N-pyridylurea chitosan quaternary ammonium salt can be used for preparing an anti-oxidant, and can be widely used in the fields of biology, medicine, food and chemical industry, the biological activity such as antioxidant activity is increased, the preparation raw materials are easily available, the preparation is simple, the condition is mild, and the preparation method establishes a base for application and exploitation for high-value chitosan.
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