The invention discloses amino chitin as well as a preparation method and an application thereof. Chitin, sodium hydroxide, a chloroform solution of p toluene sulfonyl chloride, sodium azide, dimethyl sulfoxide, and triphenyl phosphine are used as raw materials; chitin is firstly used as a raw material, p toluene sulfonyl chloride is added for preparing sulfonylated chitin, sulfonylated chitin and sodium azide are reacted, and chitin azide is obtained; an azide group is reduced in order to obtain amino chitin. The amino chitin is advantageous in that content of amino on a sugar skeleton is increased, anti-microbial determination results show that anti-microbial activity of amino chitin is obviously higher than anti-microbial activity of chitin, and effects of the product for inhibiting partial fungi approach to positive control, and the product can be widely applied to pesticide and other fields.
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