本发明涉及基因工程技术领域，具体涉及一种杜氏盐藻叶绿体同源重组空载体及其应用。载体包括启动子、终止子，所述重组空载体含SEQ ID NO : 1所示碱基序列的上游同源臂和SEQ ID NO : 2所示碱基序列的下游同源臂，同源臂之间插入与至少一个外源基因构成多顺反子结构的SEQ ID NO : 5所示的碱基序列。采用本发明的杜氏盐藻叶绿体稳定表达系统, 可实现多个外源基因在叶绿体中稳定表达。
其他摘要
Provided are a homologous recombination empty vector for dunaliella salina chloroplasts and an application thereof. The vector comprises a promoter, a terminator, an upstream homologous arm having a base sequence represented by SEQ ID NO : 1, and a downstream homologous arm having a base sequence represented by SEQ ID NO : 2. A base sequence represented by SEQ ID NO : 5 that forms a polycistronic structure with at least one exogenous gene is inserted between the homologous arms. The vector can implement stable expression of multiple exogenous genes in chloroplasts.
; 林彬; 秦松
修改评论