The invention belongs to the technical field of biological products, and specifically relates to a collection method for urechis unicinctus mucus and an application of the collected mucus. The collection method includes the following steps : (1) a step of the low temperature treatment of urechis unicinctus; (2) a step of microcurrent stimulation and body surface mucus collecting; and (3) a step offreeze-drying. The provided urechis unicinctus body surface mucus has bacteriostatic action and can be applied to antibacterial and antibacterial biological products.
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