本发明涉及基因工程技术领域，具体涉及一种紫球藻叶绿体同源重组空载体及其应用。载体包括紫球藻叶绿体基因组上SEQ ID NO : 1所示碱基序列的上游同源臂和SEQ ID NO : 2所示碱基序列的下游同源臂，SEQ ID NO : 3、SEQ ID NO : 4所示碱基序列的启动子，SEQ ID NO : 5、SEQ ID NO : 6所示碱基序列的终止子，启动子和终止子之间插入与至少一个外源基因构成多顺反子结构的SEQ ID NO : 7所示的碱基序列。采用本发明的紫球藻叶绿体同源重组空载体可实现多个外源基因在叶绿体中稳定表达。
其他摘要
The invention relates to the technical field of gene engineering, and particularly relates to a porphyridium chloroplast homologous recombination empty vector and application thereof. The vector comprises an upstream homologous arm, a downstream homologous arm, promoters and terminators, wherein a base sequence of the upstream homologous arm is shown in SEQ ID NO : 1 of a porphyridium chloroplast gene group, a base sequence of the downstream homologous arm is shown in SEQ ID NO : 2, base sequences of the promoters are shown in SEQ ID NO : 3 and SEQ ID NO : 4, base sequences of the terminators are shown in SEQ ID NO : 5 and SEQ ID NO : 6; and a corresponding base sequence which can form a multicistron structure with at least one exogenous gene and is shown in SEQ ID NO : 7 is inserted between the corresponding promoter and the corresponding terminator. By adopting the porphyridium chloroplast homologous recombination empty vector disclosed by the invention, stable expression of multiple exogenous genes in the chloroplast can be realized.
; 甄张赫; 王康; 任家利; 秦松
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