本发明涉及基因工程技术领域，具体涉及一种普通小球藻叶绿体同源重组空载体及其应用。重组空载体包括上下游同源臂，以及同源臂间设至少一个启动子和终止子，启动子和终止子之间插入与至少一个外源基因构成多顺反子结构的SEQ ID NO : 7所示的碱基序列；其中，上游同源臂含SEQ ID NO : 1所示碱基序列，下游同源臂含SEQ ID NO : 2所示碱基序列。采用本发明的普通小球藻叶绿体稳定表达系统, 可实现多个外源基因在叶绿体中稳定表达。
其他摘要
The invention relates to the technical field of gene engineering, in particular to a chlorella vulgaris chloroplast homologous recombinant empty vector and application thereof. The recombinant empty vector comprises upstream and downstream homologous arms, at least one promoter and at least one terminator are arranged between the upstream and downstream homologous arms, and a base sequence which is as shown in SEQ ID NO : 7 and forms a polycistron structure with at least one exogenous gene is inserted between the promoter and the terminator; and the upstream homologous arm contains a base sequence shown as SEQ ID NO : 1, and the downstream homologous arm contains a base sequence shown as SEQ ID NO : 2. By adopting a chlorella vulgaris chloroplast stable expression system disclosed by the invention, stable expression of a plurality of exogenous genes in chloroplast can be realized.
; 王康; 任家利; 秦松
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