本发明涉及基因工程技术领域，具体涉及一种富脂微拟球藻叶绿体转基因系统及其应用。该系统的载体包括启动子、终止子，以及SEQ ID NO : 1所示碱基序列的上游同源臂和SEQ ID NO : 2所示碱基序列的下游同源臂，同源臂之间插入与至少一个外源基因构成多顺反子结构的SEQ ID NO : 7所示的碱基序列。采用本发明的富脂微拟球藻叶绿体稳定表达系统, 可实现多个外源基因在叶绿体中稳定表达。
其他摘要
The invention relates to the technical field of genetic engineering, in particular to a rich-lipid nannochloropsis chloroplast transgenic system and application thereof. A carrier of the system comprises at least one promoter, a terminator, an upstream homologous arm and a downstream homologous arm, wherein the upstream homologous arm has a base sequence shown as SEQ ID NO : 1; the downstream homologous arm has a base sequence shown as SEQ ID NO : 2; and a base sequence, shown as SEQ ID NO : 7, forming a polycistron structure with at least one exogenous gene is inserted between the homologous arms.By using the rich-lipid nannochloropsis chloroplast transgenic system provided by the invention, the stable expression of a plurality of exogenous genes in chloroplasts can be realized.
; 王康; 任家利; 王寅初
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