The invention belongs to the technical field of biological products, and particularly relates to a preparation method and application of urechis unicinctus autolytic enzyme. The preparation method comprises the steps of taking urechis unicinctus internal organs, performing cleaning with bacteria-free water, performing homogenization, adding PBS, performing leaching, performing loading in low-temperature environment, performing standing overnight, then performing centrifugation, and collecting supernatant; performing gradient precipitating in low-temperature environment with a saturated ammonium sulfate solution to obtain protein precipitate, performing resolvation with PBS buffer liquid, and performing dialysis to obtain an autolytic enzyme crude enzyme solution; and performing column chromatography and separation and purification on the crude enzyme solution to obtain the urechis unicinctus autolytic enzyme. The urechis unicinctus autolytic enzyme prepared by the method has favorablethermal stability, has favorable wall-breaking effects on algal cells of chlorella, and can be applied to extraction of active substances in aquatic animals and processing by-products, and productionand processing of foods including algae, so as to improve degradation effects and reduce production cost.
; 吕慧超; 秦松
修改评论