The utility model relates to a three-dimensional circulating fluctuation type runway pool for culturing microalgae, comprises a first frame body and a second frame body, wherein the first frame body is provided with a plurality of layers of inclined first runways, multiple layers of inclined second runways are arranged on the second frame body, the first runway and the second runway are staggered along the height direction and are opposite in inclination direction, first buffer grooves are arranged at two ends of the first runway, second buffer grooves are arranged at two ends of the second runway, and the first buffer groove is communicated with the second buffer groove adjacent to the same side, The output end of the bottommost runway is provided with a sealed buffer tank, the buffer tank is connected with the input end of a pump, the output end of the pump is connected with the uppermost runway through a pipeline, aeration pipes are respectively arranged in the first buffer tank, the second buffer tank and the buffer tank, and the first runway, the second runway, the first buffer tank and the second buffer tank are all sealed. The utility model realizes multi-layer arrangement and circulating gradient self-flow on the same land area, saves the soil area and improves the production efficiency at the same time.
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