The invention discloses a stem cell-loaded fish collagen wound repairing sponge and a preparation method thereof. The preparation method comprises the following steps : Preparing fish collagen wound repair sponge-allowing the fish collagen wound repair sponge to absorb a complete cell culture medium-preparing a stem cell suspension-injecting stem cells into the fish collagen wound repair sponge and continuously providing the fish collagen wound repair sponge with a complete cell culture medium-combining the stem cells and the fish collagen wound Repair sponge co-culture-mild dehydration treatment. The present invention loads stem cell into fish collagen wound repairing sponge, The stem cells are distributed in the whole fish collagen wound repair sponge and grow, crawl and divide in the whole fish collagen wound repair sponge, and finally the whole fish collagen wound repair sponge is filled with the stem cells, so that the stem cells are induced to differentiate on the wound surface after a later repair material is implanted, and a targeted effect is achieved.
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