The present invention discloses a fish decalcified bone matrix and its preparation method. The preparation method comprises the following steps : placing fresh fish bone in a reaction kettle, The following treatment is carried out in sequence in the reaction kettle : stirring sodium chloride solution at 4 ℃ for 8 hr; stirring SDS solution at 4 ℃ for 8 hr; washing with pure water; stirring with glycerol at 4 ℃ for 8 hr; washing with pure water; stirring with hydrogen peroxide solution at 37 ℃ for 8 hr; washing with pure water; stirring with absolute ethyl ether at 4 ℃ for 8 hr; stirring with protease solution at 4 ℃ for 0.5 hr; washing with pure water; dehydrating with absolute ethyl alcohol; The dehydrated fish bone is taken out from the reaction kettle, and the fish decalcified bone matrix is obtained after freeze drying. The fish decalcified bone matrix prepared by the invention completely reserves the spatial structure of the fish bone, has good biocompatibility, biodegradability and bone repair capability, has no risk of co-illness of people and animals, has good mechanical strength after being crushed and remolded, and can be used for repairing various non-bearing bone defects and assisting in repairing the bearing bone defects. The fish decalcified bone matrix provided by the invention has the advantages of good biocompatibility, biodegradability and bone repair capability.
; 王蕾; 李文军
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