Bisphenol A (BPA) Directly Activates the G Protein-Coupled Estrogen Receptor 1 and Triggers the Metabolic Disruption in the Gonadal Tissue of Apostichopus japonicus

doi:10.3390/biology12060798

	Bisphenol A (BPA) Directly Activates the G Protein-Coupled Estrogen Receptor 1 and Triggers the Metabolic Disruption in the Gonadal Tissue of Apostichopus japonicus
	Yuan, Jieyi 1; Yang, Jingwen 1; Xu, Xiuwen 1; Wang, Zexianghua 1; Jiang, Zhijing 1; Ye, Zhiqing 1; Ren, Yucheng 1; Wang, Qing2 ; Wang, Tianming 1
发表期刊	BIOLOGY-BASEL
	2023-06-01
卷号	12 期号:6 页码:19
关键词	Apostichopus japonicus reproduction estradiol receptor Bisphenol A (BPA) metabolic disruption
DOI	10.3390/biology12060798
通讯作者	Wang, Qing(qingwang@yic.ac.cn) ; Wang, Tianming(wangtianming@zjou.edu.cn)
英文摘要	Simple Summary Apostichopus japonicus is a temperate marine invertebrate and an economically important aquatic echinoderm species in China. As a benthic organism, sea cucumbers feed on small benthic particulate matter and are easily affected by pollutants. Therefore, for conservation purposes, it is important to elucidate the effects of specific pollutants on these animals. Bisphenol A (BPA) has been identified as an endocrine disruptor that functions as an estrogen analog and typically causes reproductive toxicity by interfering with the endocrine system. We identified the G protein-coupled estrogen receptor 1 in A. japonicus and found that both BPA and estradiol could activate the estrogen-receptor-mediated mitogen-activated protein kinase signaling pathway. Using biological approaches, we have confirmed the estrogen-analog bioactivity of BPA on sea cucumbers by direct interaction with the estrogen receptor. We also determined that BPA affects the reproduction of sea cucumbers by metabolic disruption in ovary tissue. The sea cucumber, Apostichopus japonicus, is a marine benthic organism that feeds on small benthic particulate matter and is easily affected by pollutants. Bisphenol A (BPA, 4,4 & PRIME;-isopropylidenediphenol) has been identified as an endocrine disruptor. It is ubiquitously detectable in oceans and affects a variety of marine animals. It functions as an estrogen analog and typically causes reproductive toxicity by interfering with the endocrine system. To comparatively analyze the reproductive effects of estradiol (E-2) and BPA on sea cucumbers, we identified a G protein-coupled estrogen receptor 1 (GPER1) in A. japonicus and investigated its effects on reproduction. The results showed that BPA and E-2 exposure activated A. japonicus AjGPER1, thereby mediating the mitogen-activated protein kinase signaling pathways. High-level expression of AjGPER1 in the ovarian tissue was confirmed by qPCR. Furthermore, metabolic changes were induced by 100 nM (22.83 & mu;g/L) BPA exposure in the ovarian tissue, leading to a notable increase in the activities of trehalase and phosphofructokinase. Overall, our findings suggest that AjGPER1 is directly activated by BPA and affects sea cucumber reproduction by disrupting ovarian tissue metabolism, suggesting that marine pollutants pose a threat to the conservation of sea cucumber resources.
资助机构	National Natural Science Foundation of China ; Pioneer and Leading Goose Ramp;D Program of Zhejiang
收录类别	SCI
语种	英语
关键词[WOS]	GENE-EXPRESSION ; TOXICITY ; GPR30 ; GPER ; PREDICTION ; EXPOSURE ; RELEASE ; FISH ; PCR
研究领域[WOS]	Life Sciences & Biomedicine - Other Topics
WOS记录号	WOS:001016927900001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/33334
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Wang, Qing; Wang, Tianming
作者单位	1.Zhejiang Ocean Univ, Marine Sci & Technol Coll, Natl Engn Res Ctr Marine Facil Aquaculture, Zhoushan 316022, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresour, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Jieyi,Yang, Jingwen,Xu, Xiuwen,et al. Bisphenol A (BPA) Directly Activates the G Protein-Coupled Estrogen Receptor 1 and Triggers the Metabolic Disruption in the Gonadal Tissue of Apostichopus japonicus[J]. BIOLOGY-BASEL,2023,12(6):19.
APA	Yuan, Jieyi.,Yang, Jingwen.,Xu, Xiuwen.,Wang, Zexianghua.,Jiang, Zhijing.,...&Wang, Tianming.(2023).Bisphenol A (BPA) Directly Activates the G Protein-Coupled Estrogen Receptor 1 and Triggers the Metabolic Disruption in the Gonadal Tissue of Apostichopus japonicus.BIOLOGY-BASEL,12(6),19.
MLA	Yuan, Jieyi,et al."Bisphenol A (BPA) Directly Activates the G Protein-Coupled Estrogen Receptor 1 and Triggers the Metabolic Disruption in the Gonadal Tissue of Apostichopus japonicus".BIOLOGY-BASEL 12.6(2023):19.