Norgestrel causes digestive gland injury in the clam Mactra veneriformis: An integrated histological, transcriptomics, and metabolomics study

doi:10.1016/j.scitotenv.2023.162110

	Norgestrel causes digestive gland injury in the clam Mactra veneriformis: An integrated histological, transcriptomics, and metabolomics study
	Zhao, Xiaoran 1; Wang, Qing2 ; Li, Xiangfei 1; Xu, Hua 3; Ren, Chuanbo 4; Yang, Yanyan 4; Xu, Shuhao 1; Wei, Guoxing 1; Duan, Yujun 1; Tan, Zhitao 1; Fang, Yan 1
发表期刊	SCIENCE OF THE TOTAL ENVIRONMENT
ISSN	0048-9697
	2023-05-01
卷号	871 页码:13
关键词	Progestin Histology Transcriptome Metabolome Bivalve
DOI	10.1016/j.scitotenv.2023.162110
通讯作者	Fang, Yan(fly12689@163.com)
英文摘要	The potential adverse effects of progestins on aquatic organisms, especially non-target species, are of increasing concern worldwide. However, the effect and mechanism of progestin toxicity on aquatic invertebrates remain largely unexplored. In the present study, clams Mactra veneriformis were exposed to norgestrel (NGT, 0,10, and 1000 ng/L), the dominant progestin detected in the aquatic environment, for 21 days. NGT accumulation, histology, transcriptome, and metabolome were assessed in the digestive gland. The bioconcentration factor (BCF) was 386 and 268 in the 10 ng/L NGT group and 1000 ng/L NGT group, respectively, indicating efficient accumulation of NGT in the clams. Histological analysis showed that NGT led to the swelling of epithelial cells and blurring of the basement membrane in the digestive gland. Differentially-expressed genes and KEGG pathway enrichment analysis using a transcriptomic approach suggested that NGT primarily disturbed the detoxification system, antioxidant defense, carbohydrate and amino acid metabolism, and steroid hormone metabolism, which was consistent with the metabolites analyzed using a metabolomic approach. Furthermore, we speculated that the oxidative stress caused by NGT resulted in histological damage to the digestive gland. This study showed that NGT caused adverse effects in the clams and sheds light on the mechanisms of progestin interference in aquatic invertebrates.
资助机构	National Natural Science Founda- tion of China ; National Key Research and Devel- opment Program of China ; Ludong University Research Funding
收录类别	SCI
语种	英语
关键词[WOS]	MOSQUITOFISH GAMBUSIA-AFFINIS ; AFFECTS SEX-DIFFERENTIATION ; ACUTE REGULATORY PROTEIN ; MEDROXYPROGESTERONE ACETATE ; MYTILUS-GALLOPROVINCIALIS ; BIOTRANSFORMATION ENZYMES ; STEROID-LEVELS ; LIFE-HISTORY ; EXPOSURE ; EXPRESSION
研究领域[WOS]	Environmental Sciences & Ecology
WOS记录号	WOS:000990537800001
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/32939
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Fang, Yan
作者单位	1.Ludong Univ, Sch Agr, Yantai 264025, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresour, Yantai 264003, Peoples R China 3.Yantai Ecol Environm Monitoring Ctr, Yantai 264010, Shandong, Peoples R China 4.Shandong Marine Resource & Environm Res Inst, Shandong Prov Key Lab Restorat Marine Ecol, Yantai 264006, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Xiaoran,Wang, Qing,Li, Xiangfei,et al. Norgestrel causes digestive gland injury in the clam Mactra veneriformis: An integrated histological, transcriptomics, and metabolomics study[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2023,871:13.
APA	Zhao, Xiaoran.,Wang, Qing.,Li, Xiangfei.,Xu, Hua.,Ren, Chuanbo.,...&Fang, Yan.(2023).Norgestrel causes digestive gland injury in the clam Mactra veneriformis: An integrated histological, transcriptomics, and metabolomics study.SCIENCE OF THE TOTAL ENVIRONMENT,871,13.
MLA	Zhao, Xiaoran,et al."Norgestrel causes digestive gland injury in the clam Mactra veneriformis: An integrated histological, transcriptomics, and metabolomics study".SCIENCE OF THE TOTAL ENVIRONMENT 871(2023):13.