In vitro study of deltamethrin-induced extracellular traps in hemocytes of Ruditapes philippinarum

doi:10.1016/j.ecoenv.2023.114909

	In vitro study of deltamethrin-induced extracellular traps in hemocytes of Ruditapes philippinarum
	Han, Yijing 1; Zhang, Qianqian2,3 ; Chen, Lizhu 4; Zhao, Jianmin2,3 ; Yang, Dinglong2,3
发表期刊	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
ISSN	0147-6513
	2023-05-01
卷号	256 页码:8
关键词	Extracellular traps Deltamethrin Ruditapes philippinarum Immunotoxicity
DOI	10.1016/j.ecoenv.2023.114909
通讯作者	Yang, Dinglong(dlyang@yic.ac.cn)
英文摘要	Deltamethrin (DLM), a broad-spectrum pesticide, has been proven to have toxic effects on aquatic organisms. Here, we detected the formation of extracellular traps (ETosis) formation in Manila clam (Ruditapes philip-pinarum) hemocytes stimulated by three concentrations of DLM (0.01, 0.1 and 1 mu g/mL) in vitro, and explored the underlying mechanisms induced by this pesticide. Extracellular DNA structure observation and quantitative results indicated that DLM exposure could obviously induce hemocytes ETosis, especially under high concen-tration of DLM induction. Moreover, DLM increased the levels of myeloperoxidase (MPO) and reactive oxygen species (ROS) in a dose-dependent manner, and enhanced the mRNA expression of several ROS-related genes. DPI (NADPH oxidase inhibitor) and ABAH (MPO inhibitor) could substantially inhibit DLM-induced extracellular traps (ETs), suggesting that the induced ETs release was caused by the induction of the ROS burst and MPO production. In addition, three concentrations of DLM-induced ETs were also accompanied by mitochondrial dysfunction, such as increasing the production of mitochondrial ROS, leading to a decrease in mitochondrial membrane potential (MMP) and activation of mitochondrial permeability transition pore (MPTP). Taken together, these results will shed new light on the immunotoxicity of DLM in clams and perhaps lays the foun-dation for health assessment in bivalves.
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province ; Yantai Science and Technology Development Project ; Youth Innovation Promotion Associ- ation of CAS
收录类别	SCI
语种	英语
关键词[WOS]	FRESH-WATER MUSSEL ; OXIDATIVE STRESS ; NEUTROPHIL ELASTASE ; PESTICIDE POLLUTION ; MITOCHONDRIAL-DNA ; ACUTE TOXICITY ; RELEASE ; HISTONES ; DEATH ; BIOMARKERS
研究领域[WOS]	Environmental Sciences & Ecology ; Toxicology
WOS记录号	WOS:000982720300001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/32903
专题	中国科学院海岸带环境过程与生态修复重点实验室支撑部门_中国科学院牟平海岸带环境综合试验站
通讯作者	Yang, Dinglong
作者单位	1.Ludong Univ, Sch Agr, Yantai 264025, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Shandong, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China 4.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Han, Yijing,Zhang, Qianqian,Chen, Lizhu,et al. In vitro study of deltamethrin-induced extracellular traps in hemocytes of Ruditapes philippinarum[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2023,256:8.
APA	Han, Yijing,Zhang, Qianqian,Chen, Lizhu,Zhao, Jianmin,&Yang, Dinglong.(2023).In vitro study of deltamethrin-induced extracellular traps in hemocytes of Ruditapes philippinarum.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,256,8.
MLA	Han, Yijing,et al."In vitro study of deltamethrin-induced extracellular traps in hemocytes of Ruditapes philippinarum".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 256(2023):8.