Anti-stokes fluorescence of phycobilisome and its complex with the orange carotenoid protein

doi:10.1016/j.bbabio.2023.149014

	Anti-stokes fluorescence of phycobilisome and its complex with the orange carotenoid protein
	Zlenko, Dmitry, V 1,2; Protasova, Elena A.1; Tsoraev, Georgy, V 1; Sluchanko, Nikolai N.3; Cherepanov, Dmitry A.4,5; Friedrich, Thomas 6; Ge, Baosheng 7; Qin, Song7,8 ; Maksimov, Eugene G.1; Rubin, Andrew B.1
发表期刊	BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
ISSN	0005-2728
	2024
卷号	1865 期号:1 页码:9
关键词	Anti-Stokes fluorescence Phycobilisomes Photoprotection Orange carotenoid protein Excitation energy transfer
DOI	10.1016/j.bbabio.2023.149014
通讯作者	Maksimov, Eugene G.(emaksimoff@yandex.ru)
英文摘要	Phycobilisomes (PBSs) are giant water-soluble light-harvesting complexes of cyanobacteria and red algae, consisting of hundreds of phycobiliproteins precisely organized to deliver the energy of absorbed light to chlorophyll chromophores of the photosynthetic electron-transport chain. Quenching the excess of excitation energy is necessary for the photoprotection of photosynthetic apparatus. In cyanobacteria, quenching of PBS excitation is provided by the Orange Carotenoid Protein (OCP), which is activated under high light conditions. In this work, we describe parameters of anti-Stokes fluorescence of cyanobacterial PBSs in quenched and unquenched states. We compare the fluorescence readout from entire phycobilisomes and their fragments. The obtained results revealed the heterogeneity of conformations of chromophores in isolated phycobiliproteins, while such heterogeneity was not observed in the entire PBS. Under excitation by low-energy quanta, we did not detect a significant uphill energy transfer from the core to the peripheral rods of PBS, while the one from the terminal emitters to the bulk allophycocyanin chromophores is highly probable. We show that this direction of energy migration does not eliminate fluorescence quenching in the complex with OCP. Thus, long-wave excitation provides new insights into the pathways of energy conversion in the phycobilisome.
资助机构	Russian Science Foundation ; National Natural Science Foundation of China ; Ministry of Science and Higher education of the Russian Federation
收录类别	SCI
语种	英语
关键词[WOS]	SYNECHOCYSTIS PCC 6803 ; LIGHT-HARVESTING ANTENNA ; ALLOPHYCOCYANIN-B ; COMBINATIONAL BIOSYNTHESIS ; MOLECULAR ARCHITECTURE ; ENERGY-TRANSFER ; CORE ; MUTANT ; PHOTOPROTECTION ; PHYCOCYANIN
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:001083773600001
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/32819
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Maksimov, Eugene G.
作者单位	1.Lomonosov Moscow State Univ, Fac Biol, Moscow 119991, Russia 2.Russian Acad Sci, AN Severtsov Inst Ecol & Evolut, Moscow, Russia 3.Russian Acad Sci, Fed Res Ctr Biotechnol, AN Bach Inst Biochem, Moscow, Russia 4.Russian Acad Sci, NN Semenov Fed Res Ctr Chem Phys, Moscow 142432, Russia 5.Lomonosov Moscow State Univ, AN Belozersky Inst Phys Chem Biol, Moscow 119991, Russia 6.Tech Univ Berlin, Inst Chem PC 14, D-10623 Berlin, Germany 7.China Univ Petr Huadong, Coll Chem Engn, Qingdao 266580, Peoples R China 8.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Zlenko, Dmitry, V,Protasova, Elena A.,Tsoraev, Georgy, V,et al. Anti-stokes fluorescence of phycobilisome and its complex with the orange carotenoid protein[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2024,1865(1):9.
APA	Zlenko, Dmitry, V.,Protasova, Elena A..,Tsoraev, Georgy, V.,Sluchanko, Nikolai N..,Cherepanov, Dmitry A..,...&Rubin, Andrew B..(2024).Anti-stokes fluorescence of phycobilisome and its complex with the orange carotenoid protein.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1865(1),9.
MLA	Zlenko, Dmitry, V,et al."Anti-stokes fluorescence of phycobilisome and its complex with the orange carotenoid protein".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1865.1(2024):9.