Golgi fucosyltransferase 1 reveals its important role in alpha-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum

doi:10.1186/s12934-022-02000-2

	Golgi fucosyltransferase 1 reveals its important role in alpha-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum
	Xie, Xihui 1; Yang, Jianchao 2; Du, Hong 1; Chen, Jichen 1; Sanganyado, Edmond 1; Gong, Yangmin 1; Du, Hua 1; Chen, Weizhou 1; Liu, Zhengyi3 ; Liu, Xiaojuan 1
发表期刊	MICROBIAL CELL FACTORIES
	2023-01-07
卷号	22 期号:1 页码:18
关键词	Phaeodactylum tricornutum Glycoprotein Fucosyltransferase N-glycan Microbial cell factory
DOI	10.1186/s12934-022-02000-2
通讯作者	Du, Hong(hdu@stu.edu.cn) ; Liu, Xiaojuan(liuxiaojuan@stu.edu.cn)
英文摘要	Phaeodactylum tricornutum (Pt) is a critical microbial cell factory to produce a wide spectrum of marketable products including recombinant biopharmaceutical N-glycoproteins. N-glycosylation modification of proteins is important for their activity, stability, and half-life, especially some special modifications, such as fucose-modification by fucosyltransferase (FucT). Three PtFucTs were annotated in the genome of P. tricornutum, PtFucT1 was located on the medial/trans-Golgi apparatus and PtFucT2-3 in the plastid stroma. Algal growth, biomass and photosynthesis efficiency were significantly inhibited in a knockout mutant of PtFucT1 (PtFucT1-KO). PtFucT1 played a role in non-core fucose modification of N-glycans. The knockout of PtFucT1 might affect the activity of PtGnTI in the complex and change the complex N-glycan to mannose type N-glycan. The study provided critical information for understanding the mechanism of protein N-glycosylation modification and using microalgae as an alternative ecofriendly cell factory to produce biopharmaceuticals.
资助机构	Special fund for science and technology of Guangdong Province ; Natural Science Foundation of Guangdong Province ; 2020 Li Ka Shing Foundation Cross-Disciplinary Research Grant, China ; Program for University Innovation Team of Guangdong Province
收录类别	SCI
语种	英语
关键词[WOS]	CHLAMYDOMONAS-REINHARDTII ; GLYCOSYLATION PATHWAY ; MATURATION ; MUTANTS
研究领域[WOS]	Biotechnology & Applied Microbiology
WOS记录号	WOS:000910866800001
引用统计	被引频次：3[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/32547
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Du, Hong; Liu, Xiaojuan
作者单位	1.Shantou Univ, Inst Marine Sci, Coll Sci, STU UNIVPM Joint Algal Res Ctr,Guangdong Prov Key, Shantou 515063, Guangdong, Peoples R China 2.Yantai Acad Agr Sci, Yantai 265500, Shandong, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Xie, Xihui,Yang, Jianchao,Du, Hong,et al. Golgi fucosyltransferase 1 reveals its important role in alpha-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum[J]. MICROBIAL CELL FACTORIES,2023,22(1):18.
APA	Xie, Xihui.,Yang, Jianchao.,Du, Hong.,Chen, Jichen.,Sanganyado, Edmond.,...&Liu, Xiaojuan.(2023).Golgi fucosyltransferase 1 reveals its important role in alpha-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum.MICROBIAL CELL FACTORIES,22(1),18.
MLA	Xie, Xihui,et al."Golgi fucosyltransferase 1 reveals its important role in alpha-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum".MICROBIAL CELL FACTORIES 22.1(2023):18.