Golgi fucosyltransferase 1 reveals its important role in alpha-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum | |
Xie, Xihui1; Yang, Jianchao2; Du, Hong1; Chen, Jichen1; Sanganyado, Edmond1; Gong, Yangmin1; Du, Hua1; Chen, Weizhou1; Liu, Zhengyi3![]() | |
发表期刊 | MICROBIAL CELL FACTORIES
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2023-01-07 | |
卷号 | 22期号:1页码:18 |
关键词 | Phaeodactylum tricornutum Glycoprotein Fucosyltransferase N-glycan Microbial cell factory |
DOI | 10.1186/s12934-022-02000-2 |
通讯作者 | Du, Hong(hdu@stu.edu.cn) ; Liu, Xiaojuan(liuxiaojuan@stu.edu.cn) |
英文摘要 | Phaeodactylum tricornutum (Pt) is a critical microbial cell factory to produce a wide spectrum of marketable products including recombinant biopharmaceutical N-glycoproteins. N-glycosylation modification of proteins is important for their activity, stability, and half-life, especially some special modifications, such as fucose-modification by fucosyltransferase (FucT). Three PtFucTs were annotated in the genome of P. tricornutum, PtFucT1 was located on the medial/trans-Golgi apparatus and PtFucT2-3 in the plastid stroma. Algal growth, biomass and photosynthesis efficiency were significantly inhibited in a knockout mutant of PtFucT1 (PtFucT1-KO). PtFucT1 played a role in non-core fucose modification of N-glycans. The knockout of PtFucT1 might affect the activity of PtGnTI in the complex and change the complex N-glycan to mannose type N-glycan. The study provided critical information for understanding the mechanism of protein N-glycosylation modification and using microalgae as an alternative ecofriendly cell factory to produce biopharmaceuticals. |
资助机构 | Special fund for science and technology of Guangdong Province ; Natural Science Foundation of Guangdong Province ; 2020 Li Ka Shing Foundation Cross-Disciplinary Research Grant, China ; Program for University Innovation Team of Guangdong Province |
收录类别 | SCI |
语种 | 英语 |
关键词[WOS] | CHLAMYDOMONAS-REINHARDTII ; GLYCOSYLATION PATHWAY ; MATURATION ; MUTANTS |
研究领域[WOS] | Biotechnology & Applied Microbiology |
WOS记录号 | WOS:000910866800001 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.yic.ac.cn/handle/133337/32547 |
专题 | 海岸带生物学与生物资源利用重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
通讯作者 | Du, Hong; Liu, Xiaojuan |
作者单位 | 1.Shantou Univ, Inst Marine Sci, Coll Sci, STU UNIVPM Joint Algal Res Ctr,Guangdong Prov Key, Shantou 515063, Guangdong, Peoples R China 2.Yantai Acad Agr Sci, Yantai 265500, Shandong, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China |
推荐引用方式 GB/T 7714 | Xie, Xihui,Yang, Jianchao,Du, Hong,et al. Golgi fucosyltransferase 1 reveals its important role in alpha-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum[J]. MICROBIAL CELL FACTORIES,2023,22(1):18. |
APA | Xie, Xihui.,Yang, Jianchao.,Du, Hong.,Chen, Jichen.,Sanganyado, Edmond.,...&Liu, Xiaojuan.(2023).Golgi fucosyltransferase 1 reveals its important role in alpha-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum.MICROBIAL CELL FACTORIES,22(1),18. |
MLA | Xie, Xihui,et al."Golgi fucosyltransferase 1 reveals its important role in alpha-1,4-fucose modification of N-glycan in CRISPR/Cas9 diatom Phaeodactylum tricornutum".MICROBIAL CELL FACTORIES 22.1(2023):18. |
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