Bait microalga harboring antimicrobial peptide for controlling Vibrio infection in Argopecten irradians aquaculture

doi:10.1016/j.aquaculture.2022.739128

	Bait microalga harboring antimicrobial peptide for controlling Vibrio infection in Argopecten irradians aquaculture
	Wang, Kang 1,3; Jiao, Xudong1 ; Chu, Jinling 1; Liu, Ping 1,4; Han, Subing 1,4; Hu, Zhangli 5; Qin, Song1,3 ; Cui, Yulin1,2
发表期刊	AQUACULTURE
ISSN	0044-8486
	2023-02-25
卷号	565 页码:9
关键词	Vibrio NZ2114 Tetraselmis subcordiformis Oral drug -delivery system Scallops
DOI	10.1016/j.aquaculture.2022.739128
通讯作者	Qin, Song(sqin@yic.ac.cn) ; Cui, Yulin(yulincui@yic.ac.cn)
英文摘要	Vibrio infection is a longstanding and serious bacterial disease of various shellfish species that has caused high mortality rates for many decades. Antibiotics can effectively prevent and control bacterial diseases. However, the long-term abuse of antibiotics exacerbates the risk of environmental pollution, which also poses a threat to food safety. In this study, transgenic lines of the marine microalga Tetraselmis subcordiformis harboring antimicrobial peptide NZ2114 were attempted to be created as an oral drug-delivery system in shellfish aquaculture. Toward this, codon-optimized nz2114 was respectively assembled into nuclear and chloroplast expression vectors of T. subcordiformis. After particle bombardment, two stable transgenic lines Y1 (with nuclear transformation) and Y2 (with chloroplast transformation) were selected, each expressing a stable transgene inheritance for at least 12 months. In vitro experiments demonstrated the significant inhibition of total protein containing NZ2114 from transgenic lines on two marine Vibrio species and Staphylococcus aureus. To test the efficacy of transgenic baits, pathogen-infected scallops (Argopecten irradians) were fed with transgenic T. subcordiformis via oral delivery. The results showed that the survival rate (after 20 days of infection) of the scallops fed transgenic T. subcordiformis was much higher than that of scallop fed with wild-type algae (95.92% versus 80.41%). In summary, this strategy offers a new, efficient, and low-cost method for controlling Vibrio in scallop aquaculture through oral drug -delivery system, which is a promising step toward the development of safe and environment-friendly antimi-crobial baits.
资助机构	National Natural Science Foundation of China ; Project of Innovation and Development of Marine Economy
收录类别	SCI
语种	英语
关键词[WOS]	PICHIA-PASTORIS ; EXPRESSION ; CHLOROPLAST ; CULTIVATION ; CHLOROPHYTA ; PLECTASIN
研究领域[WOS]	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000901253800004
引用统计	被引频次：4[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/32462
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Qin, Song; Cui, Yulin
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China 2.Binzhou Med Univ, Sch Pharm, Sch Enol, Yantai 264003, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China 5.Shenzhen Univ, Coll Life Sci & Oceanog, Shenzhen 518061, Peoples R China
推荐引用方式 GB/T 7714	Wang, Kang,Jiao, Xudong,Chu, Jinling,et al. Bait microalga harboring antimicrobial peptide for controlling Vibrio infection in Argopecten irradians aquaculture[J]. AQUACULTURE,2023,565:9.
APA	Wang, Kang.,Jiao, Xudong.,Chu, Jinling.,Liu, Ping.,Han, Subing.,...&Cui, Yulin.(2023).Bait microalga harboring antimicrobial peptide for controlling Vibrio infection in Argopecten irradians aquaculture.AQUACULTURE,565,9.
MLA	Wang, Kang,et al."Bait microalga harboring antimicrobial peptide for controlling Vibrio infection in Argopecten irradians aquaculture".AQUACULTURE 565(2023):9.