Dissolved oxygen gradient on three dimensionally printed microfluidic platform for studying its effect on fish at three levels: cell, embryo, and larva

doi:10.1007/s11356-022-23688-0

	Dissolved oxygen gradient on three dimensionally printed microfluidic platform for studying its effect on fish at three levels: cell, embryo, and larva
	Liu, Ping 1,2; Fu, Longwen2,3 ; Li, Bowei2,3 ; Man, Mingsan 2; Ji, Yunxia 1; Kang, Qi 1; Sun, Xiyan 2,3; Shen, Dazhong 1; Chen, Lingxin2,3,4
发表期刊	ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
ISSN	0944-1344
	2022-10-25
页码	12
关键词	Microfluidic chip 3D-printed devices Oxygen gradient Hypoxia Fish
DOI	10.1007/s11356-022-23688-0
通讯作者	Shen, Dazhong(dzshen@sdnu.edu.cn)
英文摘要	A simple and low-cost dissolved oxygen gradient platform of three dimensionally (3D) printed microfluidic chip was developed for cultivating cells, embryos, and larvae of fish. "Christmas tree" structure channel networks generated a dissolved oxygen gradient out of two fluids fed to the device. Polydimethylsiloxane (PDMS) membrane with high biocompatibility was used as the substrate for cell culture in the 3D-printed microfluidic chip, which made the cell analysis easy. The embryos and larvae of fish could be cultured directly in the chip, and their development can be observed in real time with a microscope. Using zebrafish as a model, we assessed the effect of different dissolved oxygen on its cells, embryos, and larvae. Hypoxia induced production of reactive oxygen species (ROS) in zebrafish cells, embryos, and larvae, eventually leading to cell apoptosis and developmental impairment. Hypoxia also increased nitric oxide content in zebrafish cells, which might be a defensive strategy to overcome the adverse effect of hypoxia in fish cells. This is the first platform that could comprehensively investigate the effects of different dissolved oxygen on fish at the cell, embryo, and larva levels, which has great potential in studying the responses of aquatic organisms under different oxygen concentrations.
资助机构	National Natural Science Foundation of China ; Shandong Provincial Natural Science Foundation Key Project ; CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences
收录类别	SCI
语种	英语
关键词[WOS]	DEVELOPMENTAL TOXICITY ; OXIDATIVE STRESS ; ZEBRAFISH LARVAE ; HYPOXIA ; CULTURE ; ADAPTATION ; GENERATION ; APOPTOSIS ; DYNAMICS ; SYSTEM
研究领域[WOS]	Environmental Sciences & Ecology
WOS记录号	WOS:000871854600006
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/31952
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Shen, Dazhong
作者单位	1.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes, Minist Educ, Jinan 250014, Peoples R China 2.Chinese Acad Sci, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 4.Qingdao Univ Technol, Sch Environm & Municipal Engn, Qingdao 266033, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ping,Fu, Longwen,Li, Bowei,et al. Dissolved oxygen gradient on three dimensionally printed microfluidic platform for studying its effect on fish at three levels: cell, embryo, and larva[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2022:12.
APA	Liu, Ping.,Fu, Longwen.,Li, Bowei.,Man, Mingsan.,Ji, Yunxia.,...&Chen, Lingxin.(2022).Dissolved oxygen gradient on three dimensionally printed microfluidic platform for studying its effect on fish at three levels: cell, embryo, and larva.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,12.
MLA	Liu, Ping,et al."Dissolved oxygen gradient on three dimensionally printed microfluidic platform for studying its effect on fish at three levels: cell, embryo, and larva".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2022):12.