Proteomic and metabolomic analysis on cadmium-induced mitochondrial toxicity in liver tissues of juvenile olive flounder Paralichthys olivaceus

doi:10.3389/fmars.2022.1041705

	Proteomic and metabolomic analysis on cadmium-induced mitochondrial toxicity in liver tissues of juvenile olive flounder Paralichthys olivaceus
	Lu, Zhen 1,2,3; Xiao, Zeyu 1,2; Wu, Sihan 1,2; Song, Jun 1,2; Peng, Xiao 1,2
发表期刊	FRONTIERS IN MARINE SCIENCE
	2022-11-25
卷号	9 页码:12
关键词	cadmium mitochondrial toxicity mitochondrial proteomics metabolomics Paralichthys olivaceus
DOI	10.3389/fmars.2022.1041705
通讯作者	Peng, Xiao(pengxiao_px@szu.edu.cn)
英文摘要	IntroductionCadmium (Cd) is one of the most dominant heavy metals in the Bohai Sea. Our previous study proved that Cd could induce gill mitochondrial toxicity in marine animals. Herein, we aimed to elucidate the toxicity mechanism of Cd on liver mitochondria, as liver is the main metabolic and detoxification organ and generally rich in mitochondria. MethodsThe mitochondrial responses induced by Cd (5 and 50 mu g/L) were characterized by observing mitochondrial morphology, measuring mitochondrial membrane potential (MMP), and proteomic and metabolomic analysis in juvenile olive flounder Paralichthys olivaceus livers. ResultsAfter water-bonre exposure for 14 days, two Cd treatments decreased MMPs significantly and caused ultrastructural-damaged mitochondria in flounder livers. NMR-based metabolomics revealed that Cd exposure mainly altered the abundances of metabolites (ATP, AMP, phosphocholine, lactate and succinate) related to energy metabolism in flounder livers. iTRAQ-based mitochondrial proteomics indicated that 27 differentially expressed proteins (DEPs) were screened out from liver mitochondria after Cd treatments. These proteins were mainly associated with energy metabolism (oxidative phosphorylation (OXPHOS) and tricarboxylic acid (TCA) cycle) and apoptosis. DiscussionThese results indicated that Cd disrupted mitochondrial morphology, energy homeostasis and apoptosis in liver mitochondria in flounder P. olivaceus. This work revealed a comprehensive view on Cd-induced mitochondrial responses in the liver tissues of flounder using an integrated proteomic and metabolomic approach.
资助机构	National Natural Science Foundation of China ; Guangdong Basic and Applied Basic Research Foundation ; China Postdoctoral Science Foundation
收录类别	SCI
语种	英语
关键词[WOS]	VIRGINICA GMELIN BIVALVIA ; ENERGY-METABOLISM ; ACCUMULATION ; SEA ; SURVIVAL ; PROTEIN ; METALS ; GENE
研究领域[WOS]	Environmental Sciences & Ecology ; Marine & Freshwater Biology
WOS记录号	WOS:000894698400001
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/31898
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Peng, Xiao
作者单位	1.Shenzhen Univ, Ctr Biomed Opt & Photon CBOP, Key Lab Optoelect Devices & Syst, Minist Educ Guangdong Prov, Shenzhen, Peoples R China 2.Shenzhen Univ, Coll Phys & Optoelect Engn, Shenzhen, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Lu, Zhen,Xiao, Zeyu,Wu, Sihan,et al. Proteomic and metabolomic analysis on cadmium-induced mitochondrial toxicity in liver tissues of juvenile olive flounder Paralichthys olivaceus[J]. FRONTIERS IN MARINE SCIENCE,2022,9:12.
APA	Lu, Zhen,Xiao, Zeyu,Wu, Sihan,Song, Jun,&Peng, Xiao.(2022).Proteomic and metabolomic analysis on cadmium-induced mitochondrial toxicity in liver tissues of juvenile olive flounder Paralichthys olivaceus.FRONTIERS IN MARINE SCIENCE,9,12.
MLA	Lu, Zhen,et al."Proteomic and metabolomic analysis on cadmium-induced mitochondrial toxicity in liver tissues of juvenile olive flounder Paralichthys olivaceus".FRONTIERS IN MARINE SCIENCE 9(2022):12.