Proteomic and metabolomic analysis on cadmium-induced mitochondrial toxicity in liver tissues of juvenile olive flounder Paralichthys olivaceus
Lu, Zhen1,2,3; Xiao, Zeyu1,2; Wu, Sihan1,2; Song, Jun1,2; Peng, Xiao1,2
Source PublicationFRONTIERS IN MARINE SCIENCE
2022-11-25
Volume9Pages:12
Keywordcadmium mitochondrial toxicity mitochondrial proteomics metabolomics Paralichthys olivaceus
DOI10.3389/fmars.2022.1041705
Corresponding AuthorPeng, Xiao(pengxiao_px@szu.edu.cn)
AbstractIntroductionCadmium (Cd) is one of the most dominant heavy metals in the Bohai Sea. Our previous study proved that Cd could induce gill mitochondrial toxicity in marine animals. Herein, we aimed to elucidate the toxicity mechanism of Cd on liver mitochondria, as liver is the main metabolic and detoxification organ and generally rich in mitochondria. MethodsThe mitochondrial responses induced by Cd (5 and 50 mu g/L) were characterized by observing mitochondrial morphology, measuring mitochondrial membrane potential (MMP), and proteomic and metabolomic analysis in juvenile olive flounder Paralichthys olivaceus livers. ResultsAfter water-bonre exposure for 14 days, two Cd treatments decreased MMPs significantly and caused ultrastructural-damaged mitochondria in flounder livers. NMR-based metabolomics revealed that Cd exposure mainly altered the abundances of metabolites (ATP, AMP, phosphocholine, lactate and succinate) related to energy metabolism in flounder livers. iTRAQ-based mitochondrial proteomics indicated that 27 differentially expressed proteins (DEPs) were screened out from liver mitochondria after Cd treatments. These proteins were mainly associated with energy metabolism (oxidative phosphorylation (OXPHOS) and tricarboxylic acid (TCA) cycle) and apoptosis. DiscussionThese results indicated that Cd disrupted mitochondrial morphology, energy homeostasis and apoptosis in liver mitochondria in flounder P. olivaceus. This work revealed a comprehensive view on Cd-induced mitochondrial responses in the liver tissues of flounder using an integrated proteomic and metabolomic approach.
Funding OrganizationNational Natural Science Foundation of China ; Guangdong Basic and Applied Basic Research Foundation ; China Postdoctoral Science Foundation
Indexed BySCI
Language英语
WOS KeywordVIRGINICA GMELIN BIVALVIA ; ENERGY-METABOLISM ; ACCUMULATION ; SEA ; SURVIVAL ; PROTEIN ; METALS ; GENE
WOS Research AreaEnvironmental Sciences & Ecology ; Marine & Freshwater Biology
WOS IDWOS:000894698400001
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/31898
Collection中国科学院海岸带环境过程与生态修复重点实验室
Corresponding AuthorPeng, Xiao
Affiliation1.Shenzhen Univ, Ctr Biomed Opt & Photon CBOP, Key Lab Optoelect Devices & Syst, Minist Educ Guangdong Prov, Shenzhen, Peoples R China
2.Shenzhen Univ, Coll Phys & Optoelect Engn, Shenzhen, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai, Peoples R China
Recommended Citation
GB/T 7714
Lu, Zhen,Xiao, Zeyu,Wu, Sihan,et al. Proteomic and metabolomic analysis on cadmium-induced mitochondrial toxicity in liver tissues of juvenile olive flounder Paralichthys olivaceus[J]. FRONTIERS IN MARINE SCIENCE,2022,9:12.
APA Lu, Zhen,Xiao, Zeyu,Wu, Sihan,Song, Jun,&Peng, Xiao.(2022).Proteomic and metabolomic analysis on cadmium-induced mitochondrial toxicity in liver tissues of juvenile olive flounder Paralichthys olivaceus.FRONTIERS IN MARINE SCIENCE,9,12.
MLA Lu, Zhen,et al."Proteomic and metabolomic analysis on cadmium-induced mitochondrial toxicity in liver tissues of juvenile olive flounder Paralichthys olivaceus".FRONTIERS IN MARINE SCIENCE 9(2022):12.
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