Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores

doi:10.1063/4.0000164

	Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores
	Tsoraev, Georgy V. V.1; Protasova, Elena A. A.1; Klimanova, Elizaveta A. A.1; Ryzhykau, Yury L. L.2,3; Kuklin, Alexander I. I.2,3; Semenov, Yury S. S.2; Ge, Baosheng 4; Li, Wenjun5 ; Qin, Song4 ; Friedrich, Thomas 6; Sluchanko, Nikolai N. N.7; Maksimov, Eugene G. G.1
发表期刊	STRUCTURAL DYNAMICS-US
	2022-09-01
卷号	9 期号:5 页码:11
DOI	10.1063/4.0000164
通讯作者	Maksimov, Eugene G. G.(emaksimoff@yandex.ru)
英文摘要	The structural organization of natural pigment-protein complexes provides a specific environment for the chromophore groups. Yet, proteins are inherently dynamic and conformationally mobile. In this work, we demonstrate the heterogeneity of chromophores of C-phycocyanin (C-PC) from Arthrospira platensis. Part of the population of trimeric C-PC is subject to spontaneous disturbances of protein-protein interactions resulting in increased conformational mobility of the chromophores. Upon fluorescence excitation in the visible range, the spectral signatures of these poorly populated states are masked by bulk chromophore states, but the former could be clearly discriminated when the fluorescence is excited by near-infrared quanta. Such selective excitation of conformationally mobile C-PC chromophores is due to the structure of their S-1 level, which is characterized by a significantly broadened spectral line. We demonstrate that the anti-Stokes C-PC fluorescence is the result of single-photon absorption. By combining spectral and structural methods, we characterize four distinct states of C-PC chromophores emitting at 620, 650, 665, and 720 nm and assigned the fast component in the anti-Stokes fluorescence decay kinetics in the range of 690-750 nm to the chromophores with increased conformational mobility. Our data suggest that the spectral and temporal characteristics of the anti-Stokes fluorescence can be used to study protein dynamics and develop methods to visualize local environment parameters such as temperature. (C) 2022 Author(s).
资助机构	Russian Science Foundation ; National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province, China ; Ministry of Science and Higher Education of the Russian Federation
收录类别	SCI
语种	英语
关键词[WOS]	CYANOBACTERIUM SYNECHOCOCCUS-VULCANUS ; ANGLE SCATTERING DATA ; ENERGY-TRANSFER ; THERMAL-STABILITY ; CROSS-SECTION ; PHYCOBILISOME ; PROTEINS ; PHYCOBILIPROTEIN ; ALLOPHYCOCYANIN ; FUNCTIONALITY
研究领域[WOS]	Chemistry ; Physics
WOS记录号	WOS:000849330000001
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/31656
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Maksimov, Eugene G. G.
作者单位	1.Lomonosov Moscow State Univ, Fac Biol, Moscow 119991, Russia 2.Moscow Inst Phys & Technol, Res Ctr Mol Mech Aging & Age Related Dis, Dolgoprudnyi 141700, Russia 3.Joint Inst Nucl Res, Frank Lab Neutron Phys, Dubna 141980, Russia 4.China Univ Petr Huadong, Coll Chem Engn, Qingdao 266580, Peoples R China 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 6.Tech Univ Berlin, Inst Chem PC 14, D-10623 Berlin, Germany 7.Russian Acad Sci, Fed Res Ctr Biotechnol, AN Bach Inst Biochem, Moscow 119071, Russia
推荐引用方式 GB/T 7714	Tsoraev, Georgy V. V.,Protasova, Elena A. A.,Klimanova, Elizaveta A. A.,et al. Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores[J]. STRUCTURAL DYNAMICS-US,2022,9(5):11.
APA	Tsoraev, Georgy V. V..,Protasova, Elena A. A..,Klimanova, Elizaveta A. A..,Ryzhykau, Yury L. L..,Kuklin, Alexander I. I..,...&Maksimov, Eugene G. G..(2022).Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores.STRUCTURAL DYNAMICS-US,9(5),11.
MLA	Tsoraev, Georgy V. V.,et al."Anti-Stokes fluorescence excitation reveals conformational mobility of the C-phycocyanin chromophores".STRUCTURAL DYNAMICS-US 9.5(2022):11.