Characterization of a Haematococcus pluvialis Diacylglycerol Acyltransferase 1 and Its Potential in Unsaturated Fatty Acid-Rich Triacylglycerol Production
Cui, Hongli1; Xu, Wenxin1; Zhu, Xiaoli2; Zhao, Chunchao1; Cui, Yulin3; Ji, Chunli1; Zhang, Chunhui1; Xue, Jinai1; Qin, Song3; Jia, Xiaoyun4; Li, Runzhi1
发表期刊FRONTIERS IN PLANT SCIENCE
ISSN1664-462X
2021-12-07
卷号12页码:16
关键词Haematococcus pluvialis type-I diacylglycerol acyltransferase function characterization esterified astaxanthin triacylglycerol genetic engineering
DOI10.3389/fpls.2021.771300
通讯作者Li, Runzhi(rli2001@126.com)
英文摘要The unicellular green alga Haematococcus pluvialis has been recognized as an industry strain to produce simultaneously esterified astaxanthin (EAST) and triacylglycerol (TAG) under stress induction. It is necessary to identify the key enzymes involving in synergistic accumulation of EAST and TAG in H. pluvialis. In this study, a novel diacylglycerol acyltransferase 1 was systematically characterized by in vivo and in silico assays. The upregulated expression of HpDGAT1 gene was positively associated with the significant increase of TAG and EAST contents under stress conditions. Functional complementation by overexpressing HpDGAT1 in a TAG-deficient yeast strain H1246 revealed that HpDGAT1 could restore TAG biosynthesis and exhibited a high substrate preference for monounsaturated fatty acyl-CoAs (MUFAs) and polyunsaturated fatty acyl-CoAs (PUFAs). Notably, heterogeneous expression of HpDGAT1 in Chlamydomonas reinhardtii and Arabidopsis thaliana resulted in a significant enhancement of total oils and concurrently a high accumulation of MUFAs- and PUFAs-rich TAGs. Furthermore, molecular docking analysis indicated that HpDGAT1 contained AST-binding sites. These findings evidence a possible dual-function role for HpDGAT1 involving in TAG and EAST synthesis, demonstrating that it is a potential target gene to enrich AST accumulation in this alga and to design oil production in both commercial algae and oil crops.
收录类别SCI
语种英语
关键词[WOS]NANNOCHLOROPSIS ; TRANSFORMATION ; ASTAXANTHIN ; FEEDSTOCKS ; MICROALGAE ; REVEALS ; GENE
研究领域[WOS]Plant Sciences
WOS记录号WOS:000743041500001
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/31439
专题海岸带生物学与生物资源利用重点实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者Li, Runzhi
作者单位1.Shanxi Agr Univ, Inst Mol Agr & Bioenergy, Coll Agr, Taigu, Peoples R China
2.Shanxi Agr Univ, Coll Plant Protect, Taigu, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai, Peoples R China
4.Shanxi Agr Univ, Coll Life Sci, Taigu, Peoples R China
推荐引用方式
GB/T 7714
Cui, Hongli,Xu, Wenxin,Zhu, Xiaoli,et al. Characterization of a Haematococcus pluvialis Diacylglycerol Acyltransferase 1 and Its Potential in Unsaturated Fatty Acid-Rich Triacylglycerol Production[J]. FRONTIERS IN PLANT SCIENCE,2021,12:16.
APA Cui, Hongli.,Xu, Wenxin.,Zhu, Xiaoli.,Zhao, Chunchao.,Cui, Yulin.,...&Li, Runzhi.(2021).Characterization of a Haematococcus pluvialis Diacylglycerol Acyltransferase 1 and Its Potential in Unsaturated Fatty Acid-Rich Triacylglycerol Production.FRONTIERS IN PLANT SCIENCE,12,16.
MLA Cui, Hongli,et al."Characterization of a Haematococcus pluvialis Diacylglycerol Acyltransferase 1 and Its Potential in Unsaturated Fatty Acid-Rich Triacylglycerol Production".FRONTIERS IN PLANT SCIENCE 12(2021):16.
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