Coupling between Ribotypic and Phenotypic Traits of Protists across Life Cycle Stages and Temperatures

doi:10.1128/Spectrum.01738-21

	Coupling between Ribotypic and Phenotypic Traits of Protists across Life Cycle Stages and Temperatures
	Zou, Songbao 2,3,4,8; Fu, Rao 2,3,5; Deng, Huiwen 1,7; Zhang, Qianqian2,3 ; Gentekaki, Eleni 6; Gong, Jun1,7
发表期刊	MICROBIOLOGY SPECTRUM
ISSN	2165-0497
	2021-12-01
卷号	9 期号:3 页码:19
关键词	cell size copy number variation growth rate life cycle warming
DOI	10.1128/Spectrum.01738-21
通讯作者	Gong, Jun(gongj27@mail.sysu.edu.cn)
英文摘要	Relationships between ribotypic and phenotypic traits of protists across life cycle stages remain largely unknown. Herein, we used single cells of two soil and two marine ciliate species to examine phenotypic and ribotypic traits and their relationships across lag, log, plateau, cystic stages and temperatures. We found that Colpoda inflata and Colpoda steinii demonstrated allometric relationships between 18S ribosomal DNA (rDNA) copy number per cell (CNPC), cell volume (CV), and macronuclear volume across all life cycle stages. Integrating previously reported data of Euplotes vannus and Strombidium sulcatum indicated taxon-dependent rDNA CNPC-CV functions. Ciliate and prokaryote data analysis revealed that the rRNA CNPC followed a unified power-law function only if the rRNA-deficient resting cysts were not considered. Hence, a theoretical framework was proposed to estimate the relative quantity of resting cysts in the protistan populations with total cellular rDNA and rRNA copy numbers. Using rDNA CNPC was a better predictor of growth rate at a given temperature than rRNA CNPC and CV, suggesting replication of redundant rDNA operons as a key factor that slows cell division. Single-cell high-throughput sequencing and analysis after correcting sequencing errors revealed multiple rDNA and rRNA variants per cell. Both encystment and temperature affected the number of rDNA and rRNA variants in several cases. The divergence of rDNA and rRNA sequence in a single cell ranged from 1% to 10% depending on species. These findings have important implications for inferring cell-based biological traits (e.g., species richness, abundance and biomass, activity, and community structure) of protists using molecular approaches. IMPORTANCE Based on phenotypic traits, traditional surveys usually characterize organismal richness, abundance, biomass, and growth potential to describe diversity, organization, and function of protistan populations and communities. The rRNA gene (rDNA) and its transcripts have been widely used as molecular markers in ecological studies of protists. Nevertheless, the manner in which these molecules relate to cellular (organismal) and physiological traits remains poorly understood, which could lead to misinterpretations of protistan diversity and ecology. The current research highlights the dynamic nature of cellular rDNA and rRNA contents, which tightly couple with multiple phenotypic traits in ciliated protists. We demonstrate that quantity of resting cysts and maximum growth rate of a population can be theoretically estimated using ribotypic trait-based models. The intraindividual sequence polymorphisms of rDNA and rRNA can be influenced by encystment and temperature, which should be considered when interpreting species-level diversity and community structure of microbial eukaryotes.
资助机构	Marine S & T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) ; Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) ; Natural Science Foundation of China ; Research Fund Program of Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering ; Key Research Program of Frontier Sciences
收录类别	SCI
语种	英语
关键词[WOS]	TIME PCR ASSAY ; RIBOSOMAL-RNA ; QUANTITATIVE PCR ; MESSENGER-RNA ; RESTING CYSTS ; GROWTH-PHASE ; COPY NUMBERS ; CILIATE ; DNA ; GENE
研究领域[WOS]	Microbiology
WOS记录号	WOS:000736124700184
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/31433
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Gong, Jun
作者单位	1.Sun Yat Sen Univ, Sch Marine Sci, Zhuhai, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Minist Agr & Rural Affairs, Key Lab Hlth Freshwater Aquaculture, Huzhou, Peoples R China 5.Shandong Acad Agr Sci, Shandong Inst Sericulture, Yantai, Peoples R China 6.Mae Fah Luang Univ, Sch Sci, Chiang Rai, Thailand 7.Southern Marine Sci & Engn Guangdong Lab, Zhuhai, Peoples R China 8.Zhejiang Inst Freshwater Fisheries, Key Lab Fish Hlth & Nutr Zhejiang Prov, Huzhou, Peoples R China
推荐引用方式 GB/T 7714	Zou, Songbao,Fu, Rao,Deng, Huiwen,et al. Coupling between Ribotypic and Phenotypic Traits of Protists across Life Cycle Stages and Temperatures[J]. MICROBIOLOGY SPECTRUM,2021,9(3):19.
APA	Zou, Songbao,Fu, Rao,Deng, Huiwen,Zhang, Qianqian,Gentekaki, Eleni,&Gong, Jun.(2021).Coupling between Ribotypic and Phenotypic Traits of Protists across Life Cycle Stages and Temperatures.MICROBIOLOGY SPECTRUM,9(3),19.
MLA	Zou, Songbao,et al."Coupling between Ribotypic and Phenotypic Traits of Protists across Life Cycle Stages and Temperatures".MICROBIOLOGY SPECTRUM 9.3(2021):19.