State of the phycobilisome determines effective absorption cross-section of Photosystem II in Synechocystis sp. PCC 6803

doi:10.1016/j.bbabio.2021.148494

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	State of the phycobilisome determines effective absorption cross-section of Photosystem II in Synechocystis sp. PCC 6803
	Protasova, Elena A.1; Antal, Taras K.1; Zlenko, Dmitry, V 1; Elanskaya, Irina, V 2; Lukashev, Evgeny P.1; Friedrich, Thomas 3; Mironov, Kirill S.4; Sluchanko, Nikolai N.5; Ge, Baosheng 6; Qin, Song6,7 ; Maksimov, Eugene G.1
发表期刊	BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
ISSN	0005-2728
	2021-12-01
卷号	1862 期号:12 页码:11
关键词	Nitrogen starvation Phycobiliproteins Photosystem II Orange Carotenoid Protein
DOI	10.1016/j.bbabio.2021.148494
通讯作者	Protasova, Elena A.(123protasovaelen@gmail.com) ; Qin, Song(sqin@yic.ac.cn)
英文摘要	Quenching of excess excitation energy is necessary for the photoprotection of light-harvesting complexes. In cyanobacteria, quenching of phycobilisome (PBS) excitation energy is induced by the Orange Carotenoid Protein (OCP), which becomes photoactivated under high light conditions. A decrease in energy transfer efficiency from the PBSs to the reaction centers decreases photosystem II (PS II) activity. However, quantitative analysis of OCPinduced photoprotection in vivo is complicated by similar effects of both photochemical and non-photochemical quenching on the quantum yield of the PBS fluorescence overlapping with the emission of chlorophyll. In the present study, we have analyzed chlorophyll a fluorescence induction to estimate the effective cross-section of PS II and compared the effects of reversible OCP-dependent quenching of PBS fluorescence with reduction of PBS content upon nitrogen starvation or mutations of key PBS components. This approach allowed us to estimate the dependency of the rate constant of PS II primary electron acceptor reduction on the amount of PBSs in the cell. We found that OCP-dependent quenching triggered by blue light affects approximately half of PBSs coupled to PS II, indicating that under normal conditions, the concentration of OCP is not sufficient for quenching of all PBSs coupled to PS II.
资助机构	Russian Science Foundation ; German Research Foundation DFG ; National Natural Science Foundation of China ; Program of the Russian Ministry of Science and Higher Education
收录类别	SCI
语种	英语
关键词[WOS]	ORANGE CAROTENOID PROTEIN ; SP PCC6803 ; NITROGEN-STARVATION ; REACTION CENTERS ; ENERGY-TRANSFER ; CHLOROPHYLL-A ; CYANOBACTERIUM ; FLUORESCENCE ; MUTANT ; CORE
研究领域[WOS]	Biochemistry & Molecular Biology ; Biophysics
WOS记录号	WOS:000712537100004
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/30721
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Protasova, Elena A.; Qin, Song
作者单位	1.Lomonosov Moscow State Univ, Fac Biol, Dept Biophys, Moscow 119991, Russia 2.Lomonosov Moscow State Univ, Fac Biol, Dept Genet, Moscow 119991, Russia 3.Tech Univ Berlin, Inst Chem, D-10623 Berlin, Germany 4.Russian Acad Sci, Timiryazev Inst Plant Physiol, Moscow 127276, Russia 5.Russian Acad Sci, Fed Res Ctr Biotechnol, AN Bach Inst Biochem, Moscow 119071, Russia 6.China Univ Petr Huadong, Coll Chem Engn, Qingdao 266580, Peoples R China 7.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Protasova, Elena A.,Antal, Taras K.,Zlenko, Dmitry, V,et al. State of the phycobilisome determines effective absorption cross-section of Photosystem II in Synechocystis sp. PCC 6803[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,2021,1862(12):11.
APA	Protasova, Elena A..,Antal, Taras K..,Zlenko, Dmitry, V.,Elanskaya, Irina, V.,Lukashev, Evgeny P..,...&Maksimov, Eugene G..(2021).State of the phycobilisome determines effective absorption cross-section of Photosystem II in Synechocystis sp. PCC 6803.BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS,1862(12),11.
MLA	Protasova, Elena A.,et al."State of the phycobilisome determines effective absorption cross-section of Photosystem II in Synechocystis sp. PCC 6803".BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS 1862.12(2021):11.