Hemocyte extracellular traps of Manila clam Ruditapes philippinarum: Production characteristics and antibacterial effects
Han, Yijing1,2,3,4; Zhang, Qianqian1,2,3; Yang, Dinglong1,2,3; Zhao, Jianmin1,2,3; Chen, Lizhu5; Yu, Daode6
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
2021-03
Volume116Pages:103953
KeywordRuditapes philippinarum Extracellular traps Bactericidal effect Reactive oxygen species Glycolysis
DOI10.1016/j.dci.2020.103953
AbstractExtracellular traps (ETs) have been found to be an important strategy of mammals to immobilize and kill invading microorganisms. In the present study, we observed the formation of ETs in the hemocytes of marine mollusks Ruditapes philippinarum in response to challenge from bacteria Vibrio anguillarum, and examined the potential factors and signaling pathways underling this process. We detected an increase of reactive oxygen species (ROS) and myeloperoxidase (MPO) production during ETosis, accompanied by significantly up-regulated expression of ROS-related and MPO genes. The suppression of ETs structures by nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor (diphenyleneiodonium chloride, DPI) and MPO inhibitor (aminobenzoic acid hydrazide, ABAH) further confirmed the essential roles ROS and MPO played in ETosis. Furthermore, ET production could be inhibited by phosphotidylinsitol-3-kinase (PI3K) inhibitor (LY294002) and extracellular regulated protein kinase (ERK) inhibitor (U0126), suggesting the idea that both the PI3K and ERK pathways were suggested to function during ETosis. In addition, the ETosis process was accompanied by enhancement of glycolysis-related enzymatic activities, e.g., pyruvate kinase (PK) and hexokinase (HK), and over-expression of the glycolysis-related genes, e.g., PK, HK and glucose transport protein (GLUT), indicating high involvement of glycolysis in the ETosis process. Furthermore, our scanning electron microscopy (SEM) observation and antibacterial activities test successfully showed the patterns how clam ETs entrapped and killed the invading V. anguillarum. Taken together, our results revealed that ETosis with bactericidal effect increased ROS, MPO and glycolysis level and carried out in a ROS-, MPO-, PI3K-ERK-dependent manner.
Funding OrganizationNational Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [41806196] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2019BD022] ; Yantai Science and Technology Development Project [2020MSGY066] ; Youth Innovation Promotion Association, Chinese Academy of Sciences [2019216]
Indexed BySCI
Language英语
WOS KeywordCAPTURE BACTERIA ; DNA TRAPS ; RELEASE ; NEUTROPHILS ; ACTIVATION ; IMMUNITY ; ROLES ; DEATH ; BURST
WOS Research AreaFisheries ; Immunology ; Veterinary Sciences ; Zoology
WOS IDWOS:000607045500001
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/30517
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Shandong, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Shandong, Peoples R China;
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China;
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
5.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China;
6.Marine Biol Inst Shandong Prov, Qingdao 266002, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Han, Yijing,Zhang, Qianqian,Yang, Dinglong,et al. Hemocyte extracellular traps of Manila clam Ruditapes philippinarum: Production characteristics and antibacterial effects[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,116:103953.
APA Han, Yijing,Zhang, Qianqian,Yang, Dinglong,Zhao, Jianmin,Chen, Lizhu,&Yu, Daode.(2021).Hemocyte extracellular traps of Manila clam Ruditapes philippinarum: Production characteristics and antibacterial effects.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,116,103953.
MLA Han, Yijing,et al."Hemocyte extracellular traps of Manila clam Ruditapes philippinarum: Production characteristics and antibacterial effects".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 116(2021):103953.
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