| Comprehensive Assessment of Nile Tilapia Skin (Oreochromis niloticus) Collagen Hydrogels for Wound Dressings | |
Ge, Baosheng1,2; Wang, Haonan1,2; Li, Jie1,2,3 ; Liu, Hengheng1,2; Yin, Yonghao1,2; Zhang, Naili4; Qin, Song3
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| 发表期刊 | MARINE DRUGS
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| 2020-04-01 | |
| 卷号 | 18期号:4页码:16 |
| 关键词 | collagen hydrogel fish skin characterization wound dressing |
| DOI | 10.3390/md18040178 |
| 通讯作者 | Ge, Baosheng(gebaosheng@upc.edu.cn) ; Zhang, Naili(znl-0@163.com) ; Qin, Song(sqin@yic.ac.cn) |
| 英文摘要 | Collagen plays an important role in the formation of extracellular matrix (ECM) and development/migration of cells and tissues. Here we report the preparation of collagen and collagen hydrogel from the skin of tilapia and an evaluation of their potential as a wound dressing for the treatment of refractory wounds. The acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted and characterized using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), differential scanning calorimetry (DSC), circular dichroism (CD) and Fourier transform infrared spectroscopy (FTIR) analysis. Both ASC and PSC belong to type I collagen and have a complete triple helix structure, but PSC shows lower molecular weight and thermal stability, and has the inherent low antigenicity. Therefore, PSC was selected to prepare biomedical hydrogels using its self-aggregating properties. Rheological characterization showed that the mechanical strength of the hydrogels increased as the PSC content increased. Scanning electron microscope (SEM) analysis indicated that hydrogels could form a regular network structure at a suitable PSC content. Cytotoxicity experiments confirmed that hydrogels with different PSC content showed no significant toxicity to fibroblasts. Skin repair experiments and pathological analysis showed that the collagen hydrogels wound dressing could significantly accelerate the healing of deep second-degree burn wounds and the generation of new skin appendages, which can be used for treatment of various refractory wounds. |
| 资助机构 | National Natural Science Foundation of China ; major basic research plan of Shandong Province ; Shandong Province Higher Educational Science and Technology Program ; Natural Science Foundation of Shandong Province |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | PEPSIN-SOLUBLE COLLAGEN ; CROSS-LINKING ; I COLLAGEN ; ACID ; FIBROBLASTS ; SCAFFOLD ; GELATIN ; EXTRACTION ; BIOCOMPATIBILITY ; BIOMATERIALS |
| 研究领域[WOS] | Pharmacology & Pharmacy |
| WOS记录号 | WOS:000533909300029 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/29059 |
| 专题 | 海岸带生物学与生物资源利用重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Ge, Baosheng; Zhang, Naili; Qin, Song |
| 作者单位 | 1.China Univ Petr East China, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China 2.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone, Yantai 264003, Peoples R China 4.Binzhou Med Univ, Sch Basic Med Sci, Dept Human Anat, 346 Guanhai Rd, Laishan 264003, Yantai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ge, Baosheng,Wang, Haonan,Li, Jie,et al. Comprehensive Assessment of Nile Tilapia Skin (Oreochromis niloticus) Collagen Hydrogels for Wound Dressings[J]. MARINE DRUGS,2020,18(4):16. |
| APA | Ge, Baosheng.,Wang, Haonan.,Li, Jie.,Liu, Hengheng.,Yin, Yonghao.,...&Qin, Song.(2020).Comprehensive Assessment of Nile Tilapia Skin (Oreochromis niloticus) Collagen Hydrogels for Wound Dressings.MARINE DRUGS,18(4),16. |
| MLA | Ge, Baosheng,et al."Comprehensive Assessment of Nile Tilapia Skin (Oreochromis niloticus) Collagen Hydrogels for Wound Dressings".MARINE DRUGS 18.4(2020):16. |
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