YIC-IR  > 海岸带生物学与生物资源利用重点实验室
Antibacterial activities and mechanisms of action of a defensin from manila clam Ruditapes philippinarum
Lv, Chengjie1,2; Han, Yijing3,5; Yang, Dinglong3,4; Zhao, Jianmin3,4; Wang, Chunlin1,2; Mu, Changkao1,2
Source PublicationFISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
2020-08-01
Volume103Pages:266-276
KeywordDefensin Ruditapes philippinarum Antimicrobial activity Membrane disruption
DOI10.1016/j.fsi.2020.05.025
Corresponding AuthorYang, Dinglong(dlyang@yic.ac.cn) ; Mu, Changkao(muchangkao@nbu.edu.cn)
AbstractDefensins represent an evolutionary ancient family of antimicrobial peptides, which played an undeniably important role in host defense. In the present study, a defensin isoform was identified and characterized from manila clam Ruditapes philippinarum (designed as Rpdef1 alpha). Multiple alignments and phylogenetic analysis suggested that Rpdef1 alpha belonged to the defensin family. Quantitative RT-PCR and immunohistochemical analysis revealed that Rpdef1 alpha transcripts and the encoding peptide were dominantly expressed in the tissues of gills and mantle. After Vibrio anguillarum challenge, the Rpdef1 alpha transcripts were significantly up-regulated in gills of clams. In addition, rRpdef1 alpha not only showed broad-spectrum antimicrobial activities towards Vibrio species, but also inhibited the formation of bacterial biofilms. Knockdown of Rpdef1 alpha transcripts caused significant increase in the cumulative mortality of manila clams post V. anguillarum challenge. Membrane integrity, scanning electron microscopy analysis and electrochemical assay indicated that rRpdef1 alpha was capable of causing bacterial membrane permeabilization and then resulted in cell death. Moreover, phagocytosis and chemotactic ability of hemocytes could be significantly enhanced after incubation with rRpdef1 alpha. Overall, these results suggested that Rpdef1 alpha could act as both antibacterial agent and opsonin to defend against the invading microorganisms in manila clam R. philippinarum.
Funding OrganizationStrategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province
Indexed BySCI
Language英语
WOS KeywordANTIMICROBIAL PEPTIDES ; ESCHERICHIA-COLI ; MOLECULAR CHARACTERIZATION ; ARTHROPOD DEFENSIN ; OYSTER ; EXPRESSION ; MUSSEL ; MEMBRANE ; IMMUNITY ; PROTEIN
WOS Research AreaFisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000540375500030
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/28710
Collection海岸带生物学与生物资源利用重点实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
Corresponding AuthorYang, Dinglong; Mu, Changkao
Affiliation1.Ningbo Univ, Sch Marine Sci, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315832, Peoples R China
2.Collaborat Innovat Ctr Zhejiang Marine High Effic, Ningbo 315832, Peoples R China
3.Chinese Acad Sci, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Lv, Chengjie,Han, Yijing,Yang, Dinglong,et al. Antibacterial activities and mechanisms of action of a defensin from manila clam Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2020,103:266-276.
APA Lv, Chengjie,Han, Yijing,Yang, Dinglong,Zhao, Jianmin,Wang, Chunlin,&Mu, Changkao.(2020).Antibacterial activities and mechanisms of action of a defensin from manila clam Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,103,266-276.
MLA Lv, Chengjie,et al."Antibacterial activities and mechanisms of action of a defensin from manila clam Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 103(2020):266-276.
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