Antibacterial activities and mechanisms of action of a defensin from manila clam Ruditapes philippinarum

doi:10.1016/j.fsi.2020.05.025

	Antibacterial activities and mechanisms of action of a defensin from manila clam Ruditapes philippinarum
	Lv, Chengjie 1,2; Han, Yijing 3,5; Yang, Dinglong3,4 ; Zhao, Jianmin3,4 ; Wang, Chunlin 1,2; Mu, Changkao 1,2
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
	2020-08-01
卷号	103 页码:266-276
关键词	Defensin Ruditapes philippinarum Antimicrobial activity Membrane disruption
DOI	10.1016/j.fsi.2020.05.025
通讯作者	Yang, Dinglong(dlyang@yic.ac.cn) ; Mu, Changkao(muchangkao@nbu.edu.cn)
英文摘要	Defensins represent an evolutionary ancient family of antimicrobial peptides, which played an undeniably important role in host defense. In the present study, a defensin isoform was identified and characterized from manila clam Ruditapes philippinarum (designed as Rpdef1 alpha). Multiple alignments and phylogenetic analysis suggested that Rpdef1 alpha belonged to the defensin family. Quantitative RT-PCR and immunohistochemical analysis revealed that Rpdef1 alpha transcripts and the encoding peptide were dominantly expressed in the tissues of gills and mantle. After Vibrio anguillarum challenge, the Rpdef1 alpha transcripts were significantly up-regulated in gills of clams. In addition, rRpdef1 alpha not only showed broad-spectrum antimicrobial activities towards Vibrio species, but also inhibited the formation of bacterial biofilms. Knockdown of Rpdef1 alpha transcripts caused significant increase in the cumulative mortality of manila clams post V. anguillarum challenge. Membrane integrity, scanning electron microscopy analysis and electrochemical assay indicated that rRpdef1 alpha was capable of causing bacterial membrane permeabilization and then resulted in cell death. Moreover, phagocytosis and chemotactic ability of hemocytes could be significantly enhanced after incubation with rRpdef1 alpha. Overall, these results suggested that Rpdef1 alpha could act as both antibacterial agent and opsonin to defend against the invading microorganisms in manila clam R. philippinarum.
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province
收录类别	SCI
语种	英语
关键词[WOS]	ANTIMICROBIAL PEPTIDES ; ESCHERICHIA-COLI ; MOLECULAR CHARACTERIZATION ; ARTHROPOD DEFENSIN ; OYSTER ; EXPRESSION ; MUSSEL ; MEMBRANE ; IMMUNITY ; PROTEIN
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000540375500030
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/28710
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者	Yang, Dinglong; Mu, Changkao
作者单位	1.Ningbo Univ, Sch Marine Sci, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315832, Peoples R China 2.Collaborat Innovat Ctr Zhejiang Marine High Effic, Ningbo 315832, Peoples R China 3.Chinese Acad Sci, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Lv, Chengjie,Han, Yijing,Yang, Dinglong,et al. Antibacterial activities and mechanisms of action of a defensin from manila clam Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2020,103:266-276.
APA	Lv, Chengjie,Han, Yijing,Yang, Dinglong,Zhao, Jianmin,Wang, Chunlin,&Mu, Changkao.(2020).Antibacterial activities and mechanisms of action of a defensin from manila clam Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,103,266-276.
MLA	Lv, Chengjie,et al."Antibacterial activities and mechanisms of action of a defensin from manila clam Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 103(2020):266-276.

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