| Establishment of a chloroplast transformation system inTisochrysis lutea | |
Bo, Yuntai1; Wang, Kang2; Wu, Yuanyuan1; Cao, Haiyang1; Cui, Yulin3 ; Wang, Lan4,5
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| 发表期刊 | JOURNAL OF APPLIED PHYCOLOGY
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| ISSN | 0921-8971 |
| 2020-07-14 | |
| 页码 | 7 |
| 关键词 | Tisochrysis lutea Genetic transformation Chloroplast Biolistic method |
| DOI | 10.1007/s10811-020-02159-4 |
| 通讯作者 | Wang, Lan(WL_312@163.com) |
| 英文摘要 | Tisochrysis luteais a haptophyte microalga commonly used as a commercial feed for juvenile fish and shellfish larvae. Genetic modification is of considerable importance for developing the potential economic value ofT. lutea. However, the genetic transformation system ofT. luteahas not yet been established, which limits functional genomic studies and strain improvement of this important microalgal species. In the current study, a chloroplast transformation vector harboring the phosphinothricin resistance gene (bar) as a selectable marker was established, and two short peptide-encoding genes (ant1 andant2) driven by the endogenouspsbAandrbcLpromoters were cloned in this vector. The recombinant plasmid was transformed using a biolistic method into thetrnI/trnAlocus of the chloroplast genome via homologous recombination. After continuous selection on phosphinothricin, the integration of foreign genes and the expression of specific products in the transformants were detected using polymerase chain reaction (PCR), Southern blotting, and western blot analysis. This is the first report of establishment of a stable transformation system inT. lutea, which is a prerequisite for functional genomics and applied research on this species. |
| 资助机构 | National Natural Science Foundation of China ; Natural Science Foundation of Shandong Province, China ; Project of Innovation & Development of Marine Economy |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CULTIVATION MODE ; CHLAMYDOMONAS ; DNA |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS记录号 | WOS:000548460700001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/28629 |
| 专题 | 海岸带生物学与生物资源利用重点实验室 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 通讯作者 | Wang, Lan |
| 作者单位 | 1.Yantai Marine Environm Monitoring Ctr Stn SOA, Yantai 264006, Peoples R China 2.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Shandong, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Shandong, Peoples R China 4.Shandong Prov Key Lab Marine Ecol & Environm & Di, Qingdao 266000, Peoples R China 5.North China Sea Marine Forecasting Ctr SOA, 27 Yunling Rd, Qingdao 266000, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bo, Yuntai,Wang, Kang,Wu, Yuanyuan,et al. Establishment of a chloroplast transformation system inTisochrysis lutea[J]. JOURNAL OF APPLIED PHYCOLOGY,2020:7. |
| APA | Bo, Yuntai,Wang, Kang,Wu, Yuanyuan,Cao, Haiyang,Cui, Yulin,&Wang, Lan.(2020).Establishment of a chloroplast transformation system inTisochrysis lutea.JOURNAL OF APPLIED PHYCOLOGY,7. |
| MLA | Bo, Yuntai,et al."Establishment of a chloroplast transformation system inTisochrysis lutea".JOURNAL OF APPLIED PHYCOLOGY (2020):7. |
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