田菁种子内生菌的分离及其对萌发的影响

	田菁种子内生菌的分离及其对萌发的影响
其他题名	Isolation of Endophytic Cultures From Sesbania cannabina Seeds and Their Effects on Germination
	张凯晔 1; 刘晓琳 2; 董小燕 2; 刘润进 4; 贺立恒 1; 解志红2
发表期刊	中国农业科技导报
ISSN	1008-0864
	2020
卷号	22.0 期号:006 页码:40-48
关键词	田菁种子内生菌 IAA 植物促生菌
英文摘要	植物-微生物联合修复是改良盐碱地的重要生物策略,其中获得有效的微生物菌株是关键所在。为了获得更多的植物促生菌菌种资源,以滩涂耐盐植物田菁种子为材料分离可培养内生菌。分离纯化得到了79株菌,主要集中分布在Bacillus、Azorhizobium、Cellulosimicrobium三个属。菌株SC45、SC59、SC60、SC24、SC5、SC17六株菌可分泌吲哚乙酸(indole-3-acetic acid,IAA)。SC45、SC59、SC60具有溶解无机磷能力,其中SC60分解无机磷能力最高为(207.9±14.29)mg·L-1,且SC45具有产铁载体能力。种子萌发试验结果显示,与对照相比,SC45、SC60、SC59、SC17对田菁种子的活力系数分别可提高13.3%、14.1%、29.9%、26.7%。且SC60和SC17菌株浸种可显著促进胚根的发育,胚根占比提高了29.5%和13%。上述结果表明,Bacillus SC60可分泌IAA和溶解无机磷,提高种子活力,促进胚根发育,可作为田菁促生菌应用于农业生产。
中文摘要	Plant-microorganism combined bioremediation is important for saline-alkali land remediation, and the key is to obtain effective microbial strain. In order to obtain more resources of plant growth promoting bacteria(PGPB), the cultivable endophytes of S. cannabina seeds were used to isolate endophytic culture. 79 strains were isolated from S. cannabina seeds, belonging to Bacillus, Azorhizobium, Cellulosimicrobium. Six strains producted IAA were selected to measure the phosphate solubilization and siderophore production. SC45, SC59, SC60 had phosphate solubilization, among them, SC60 had the highest production with(207.9±14.29) mg·L-1, and SC45 had siderophore production. Germination experiment showed that six strains could promote germination potential and seed vigor. SC45, SC60, SC59 and SC17 increased the vigor coefficient of S. cannabina seeds by 13.3%, 14.1%, 29.9% and 26.7%, respectively. Soaking seeds with SC60 and SC17 strains also promoted radicle development, and the radicle ratios increased by 29.5% and 13%. Above results showed that SC60 had the IAA activity and phosphate solubilizing activity, and could be used as plant growth-promoting bacteria to promote plant radicle development and increase seed vigor, so it could be as potential PGPB to promote S. cannabina growth.
收录类别	CSCD
语种	中文
CSCD记录号	CSCD:6741630
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/28078
专题	海岸带生物学与生物资源利用重点实验室海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位	1.山西农业大学 2.中国科学院烟台海岸带研究所 3.中科院海洋大科学研究中心 4.青岛农业大学
推荐引用方式 GB/T 7714	张凯晔,刘晓琳,董小燕,等. 田菁种子内生菌的分离及其对萌发的影响[J]. 中国农业科技导报,2020,22.0(006):40-48.
APA	张凯晔,刘晓琳,董小燕,刘润进,贺立恒,&解志红.(2020).田菁种子内生菌的分离及其对萌发的影响.中国农业科技导报,22.0(006),40-48.
MLA	张凯晔,et al."田菁种子内生菌的分离及其对萌发的影响".中国农业科技导报 22.0.006(2020):40-48.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
田菁种子内生菌的分离及其对萌发的影响.p（1461KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览请求全文