Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli
Li, Wenjun1,2; Ma, Chengbo1,3; Ge, Baosheng4; Zang, Fan1,3; Qin, Song1,2; Lin, Jian3
Source PublicationJOURNAL OF APPLIED PHYCOLOGY
ISSN0921-8971
2021-04-29
Pages11
KeywordCombinational biosynthesis Phycoerythrobilin Response surface methodology Extraction
DOI10.1007/s10811-021-02408-0
Corresponding AuthorQin, Song(sqin@yic.ac.cn) ; Lin, Jian(linjian3384@163.com)
AbstractPhycoerythrobilin (PEB) is a special pigment in red algae and cyanobacteria that plays an important role in capturing light energy. As a natural non-toxic pigment with antioxidant properties, PEB is widely used in food and cosmetics industries. At present, the presence of other pigments in red algae and cyanobacteria, such as chlorophyll and phycocyanobilin, makes the preparation of PEB complicated and costly. In this study, the PEB synthesizing enzyme genes of hox1 from Synechocystis sp. PCC6803 and pebS from Nostoc sp. PCC 7120 were co-expressed in Escherichia coli to produce recombinant PEB. In addition, single-factor and response surface methods were used to enhance the yield of recombinant PEB. Under the conditions of lactose concentration at 4 mmol L-1, induction temperature at 27 degrees C, OD600 nm of 0.9, and induction time at 14 h, the concentration of PEB reached 20.37 mg L-1. In addition, a high-purity (purity > 70%) PEB was obtained through chloroform extraction, and the purified product was verified in UV spectrometry, high-performance liquid chromatography, and mass spectrometry.
Funding OrganizationNational Natural Science Foundation of China ; Key Research and Development Program of Yantai City, Shandong, China
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Marine & Freshwater Biology
WOS IDWOS:000645498300002
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/27224
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
Corresponding AuthorQin, Song; Lin, Jian
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China
Recommended Citation
GB/T 7714
Li, Wenjun,Ma, Chengbo,Ge, Baosheng,et al. Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli[J]. JOURNAL OF APPLIED PHYCOLOGY,2021:11.
APA Li, Wenjun,Ma, Chengbo,Ge, Baosheng,Zang, Fan,Qin, Song,&Lin, Jian.(2021).Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli.JOURNAL OF APPLIED PHYCOLOGY,11.
MLA Li, Wenjun,et al."Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli".JOURNAL OF APPLIED PHYCOLOGY (2021):11.
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