| Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli | |
Li, Wenjun1,2 ; Ma, Chengbo1,3; Ge, Baosheng4; Zang, Fan1,3; Qin, Song1,2; Lin, Jian3
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| Source Publication | JOURNAL OF APPLIED PHYCOLOGY
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| ISSN | 0921-8971 |
| 2021-04-29 | |
| Pages | 11 |
| Keyword | Combinational biosynthesis Phycoerythrobilin Response surface methodology Extraction |
| DOI | 10.1007/s10811-021-02408-0 |
| Corresponding Author | Qin, Song(sqin@yic.ac.cn) ; Lin, Jian(linjian3384@163.com) |
| Abstract | Phycoerythrobilin (PEB) is a special pigment in red algae and cyanobacteria that plays an important role in capturing light energy. As a natural non-toxic pigment with antioxidant properties, PEB is widely used in food and cosmetics industries. At present, the presence of other pigments in red algae and cyanobacteria, such as chlorophyll and phycocyanobilin, makes the preparation of PEB complicated and costly. In this study, the PEB synthesizing enzyme genes of hox1 from Synechocystis sp. PCC6803 and pebS from Nostoc sp. PCC 7120 were co-expressed in Escherichia coli to produce recombinant PEB. In addition, single-factor and response surface methods were used to enhance the yield of recombinant PEB. Under the conditions of lactose concentration at 4 mmol L-1, induction temperature at 27 degrees C, OD600 nm of 0.9, and induction time at 14 h, the concentration of PEB reached 20.37 mg L-1. In addition, a high-purity (purity > 70%) PEB was obtained through chloroform extraction, and the purified product was verified in UV spectrometry, high-performance liquid chromatography, and mass spectrometry. |
| Funding Organization | National Natural Science Foundation of China ; Key Research and Development Program of Yantai City, Shandong, China |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| WOS ID | WOS:000645498300002 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.yic.ac.cn/handle/133337/27224 |
| Collection | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| Corresponding Author | Qin, Song; Lin, Jian |
| Affiliation | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Wenjun,Ma, Chengbo,Ge, Baosheng,et al. Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli[J]. JOURNAL OF APPLIED PHYCOLOGY,2021:11. |
| APA | Li, Wenjun,Ma, Chengbo,Ge, Baosheng,Zang, Fan,Qin, Song,&Lin, Jian.(2021).Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli.JOURNAL OF APPLIED PHYCOLOGY,11. |
| MLA | Li, Wenjun,et al."Biosynthesis and preparation of phycoerythrobilin in recombinant Escherichia coli".JOURNAL OF APPLIED PHYCOLOGY (2021):11. |
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| Biosynthesis and pre（1586KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | View Download | |
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