Energy transfer dynamics in B-phycoerythrin from the red alga Porphyridium purpureum
Li WJ(李文军)1; Pu, Y3; Tang, ZH4; Zhao, FL2; Xie, MY2,5; Qin, S1
Source PublicationChinese Journal of Physics
ISSN0577-9073
2020
Volume66Pages:24-35
KeywordB-phycoerythrin Energy transfer Circular dichroism spectra Ultrafast spectroscopy
MOST Discipline CataloguePhysics
DOI10.1016/j.cjph.2020.03.025
Abstract

The B-phycoerythrin hexamer (αβ)6γ of Porphyridium purpureum was isolated and purified. The absorption, circular dichroism, fluorescence and ultrafast time-resolved spectra were obtained. The results showed a double absorption peak at 545 nm and 565 nm and a shoulder peak at 498 nm, and fluorescence emission maxima at 580 nm and 620 nm were observed. The circular dichroism spectra in the near-ultraviolet region were obtained and resolved for the first time, which showed that the two peaks at 260 nm and 305 nm were considered to be correlated to phenylalanine (Phe) and tryptophan (Trp) in a conservative hydrophobic microenvironment, respectively. The circular dichroism spectra in the visible region showed that PEB139α/PEB158β and PEB82α/PEB82β existed as two exciton-coupled bilin pairs. Energy transfer within the exciton-coupled pairs was by exciton splitting, while between the exciton-coupled pairs was by Förster resonance. From the studies of the energy transfer dynamics by ultrafast time-resolved fluorescence spectroscopy, it was confirmed that the energy transfer of the B-PE hexamer had three time components of 8 ps, 60 ps, and 1200 ps. In addition, the internal energy transfer pathways of B-phycoerythrin hexamer were identified by deconvoluting the fluorescence decay curve at different detection wavelengths.

SubtypeArticle
Indexed BySCI
Language英语
WOS KeywordCRYSTAL-STRUCTURE ; C-PHYCOCYANIN ; PURIFICATION ; CRUENTUM ; PHYCOBILISOMES ; PHYCOBILIPROTEINS ; RESOLUTION ; MODEL
WOS Research AreaPhysics, Multidisciplinary
WOS IDWOS:000558937700004
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/25266
Collection海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
Corresponding AuthorXie, MY; Qin, S
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Laishan Dist 264003, Yantai, Peoples R China
2.Sun Yat Sen Univ, State Key Lab Optoelect Mat & Technol, Sch Phys, Guangzhou 510275, Guangdong, Peoples R China
3.‎ Ludong Univ, Sch Agr, Yantai 264025, Peoples R China
4.Yantai Univ, Coll Life Sci, 30 Qingquan St, Yantai 264005, Peoples R China
5.Beijing Inst Technol, Sch Informat Technol, Zhuhai 519088, Zhuhai, Peoples R China
Recommended Citation
GB/T 7714
Li WJ,Pu, Y,Tang, ZH,et al. Energy transfer dynamics in B-phycoerythrin from the red alga Porphyridium purpureum[J]. Chinese Journal of Physics,2020,66:24-35.
APA Li WJ,Pu, Y,Tang, ZH,Zhao, FL,Xie, MY,&Qin, S.(2020).Energy transfer dynamics in B-phycoerythrin from the red alga Porphyridium purpureum.Chinese Journal of Physics,66,24-35.
MLA Li WJ,et al."Energy transfer dynamics in B-phycoerythrin from the red alga Porphyridium purpureum".Chinese Journal of Physics 66(2020):24-35.
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