| Enhanced biomass and CO2 sequestration of Chlorella vulgaris using a new mixotrophic cultivation method | |
Yu, Qian; Wang, Haonan; Li, Xiao; Yin, Yonghao; Qin, Song ; Ge, Baosheng
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| 发表期刊 | PROCESS BIOCHEMISTRY
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| 2020-03 | |
| 卷号 | 90页码:168-176 |
| 关键词 | CARBON-DIOXIDE INORGANIC CARBON FLUE-GAS MICROALGAE GROWTH MARINE EXPRESSION CHALLENGES MECHANISM ANHYDRASE |
| 研究领域 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| DOI | 10.1016/j.procbio.2019.11.022 |
| 产权排序 | [Yu, Qian; Wang, Haonan; Li, Xiao; Yin, Yonghao; Ge, Baosheng] China Univ Petr East China, State Key Lab Heavy Oil Proc & Ctr Bioengn & Biot, Qingdao 266580, Peoples R China; [Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源保护实验室 |
| 英文摘要 | CO2 sequestration using microalgae has been deemed as a promising way, which can fix CO2 and simultaneously produce valuable bio-products. However, large scale open-pond production of Chlorella vulgaris is normally cultured using acetic acid as a carbon source, which is costly and shows a low efficiency in CO2 fixation. Here, a new mixotrophic culture strategy using both CO2 and acetic acid was developed and evaluated for CO2 sequestration by a C. vulgaris culture in an open pond. Our results show that the growth rate of C. vulgaris under this new mixotrophic condition reaches 0.24 g/L/d, which is higher than the 0.15 and 0.21 g/L/d of photoheterotrophic culture with acetic acids and photoautotrophic culture with CO2, respectively. The averaged CO2 fixation rate was determined as 0.29 g/L/d, which is much higher than heterotrophic method but slightly lower than photoautotrophic method. This result was further confirmed in a 125 m(2) open raceway pond. Physiological and biochemical characterization showed that the cell quality of C. vulgaris under mixotrophic conditions is better than those of photoautotrophic method and photoheterotrophic cultures. The enzyme activity assay and transcriptome sequencing analysis revealed that the metabolism of carbohydrates and amino acids was significantly enhanced under mixotrophic condition compared with other groups, which may attribute to the increased biomass and CO2 sequestration of C. vulgaris. Our results suggest that this mixotrophic strategy can be applied in large-scale cultivation of C. vulgaris for biomass production and CO2 sequestration. |
| 文章类型 | Article |
| 资助机构 | National key Research and Development Plan of China [2016YFE0106700] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21673294] ; Key Research Project of Shandong Province [2017GGX40114] ; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [17CX05013] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | CARBON-DIOXIDE ; INORGANIC CARBON ; FLUE-GAS ; MICROALGAE ; GROWTH ; MARINE ; EXPRESSION ; CHALLENGES ; MECHANISM ; ANHYDRASE |
| WOS记录号 | WOS:000526116800019 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/25074 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.China Univ Petr East China, State Key Lab Heavy Oil Proc & Ctr Bioengn & Biot, Qingdao 266580, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Qian,Wang, Haonan,Li, Xiao,et al. Enhanced biomass and CO2 sequestration of Chlorella vulgaris using a new mixotrophic cultivation method[J]. PROCESS BIOCHEMISTRY,2020,90:168-176. |
| APA | Yu, Qian,Wang, Haonan,Li, Xiao,Yin, Yonghao,Qin, Song,&Ge, Baosheng.(2020).Enhanced biomass and CO2 sequestration of Chlorella vulgaris using a new mixotrophic cultivation method.PROCESS BIOCHEMISTRY,90,168-176. |
| MLA | Yu, Qian,et al."Enhanced biomass and CO2 sequestration of Chlorella vulgaris using a new mixotrophic cultivation method".PROCESS BIOCHEMISTRY 90(2020):168-176. |
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