| A Dual Role of Amino Acids from Sesbania rostrata Seed Exudates in the Chemotaxis Response of Azorhizobium caulinodans ORS571 | |
| Liu, XL; Xie, ZH; Wang, YX; Sun, Y; Dang, XX; Sun, HS | |
| 发表期刊 | MOLECULAR PLANT-MICROBE INTERACTIONS
 |
| ISSN | 0894-0282 |
| 2019-09 | |
| 卷号 | 32期号:9页码:1134-1147 |
| 关键词 | genetics and gene regulation molecular signaling Rhizobium-legume symbiosis |
| 研究领域 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences |
| DOI | 10.1094/MPMI-03-19-0059-R |
| 产权排序 | [Liu, Xiaolin; Xie, Zhihong; Wang, Yixuan; Sun, Yu; Dang, Xiaoxiao] Chinese Acad Sci, Key Lab Coastal Biol & Bioresource Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; [Liu, Xiaolin; Wang, Yixuan; Sun, Yu; Dang, Xiaoxiao] Univ Chinese Acad Sci, Beijing, Peoples R China; [Sun, Haishuan] Shandong Huibang Bohai Agr Dev Ltd Co, Dongying, Peoples R China |
| 通讯作者 | Xie, Zhihong(zhxie@yic.ac.cn) |
| 作者部门 | 海岸带生物学与生物资源保护实验室 |
| 英文摘要 | Azorhizobium caulinodans ORS571 can induce nodule formation on the roots and the stems of its host legume, Sesbania rostrata. Plant exudates are essential in the dialogue between microbes and their host plant and, in particular, amino acids can play an important role in the chemotactic response of bacteria. Histidine, arginine, and aspartate, which are the three most abundant amino acids present in S. rostrata seed exudates, behave as chemoattractants toward A. caulinodans. A position-specific-iterated BLAST analysis of the methyl-accepting chemotaxis proteins (MCPs) (chemoreceptors) in the genome of A. caulinodans was performed. Among the 43 MCP homologs, two MCPs harboring a dCache domain were selected as possible cognate amino acid MCPs. After analysis of relative gene expression levels and construction of a gene-deleted mutant strain, one of them, AZC_0821 designed as TlpH, was confirmed to be responsible for the chemotactic response to the three amino acids. In addition, it was found that these three amino acids can also influence chemotaxis of A. caulinodans independently of the chemosensory receptors, by being involved in the increase of the expression level of several the and fla genes involved in the chemotaxis pathway and flagella synthesis. Thus, the contribution of amino acids present in seed exudates is directly related to the role as chemoattractants and indirectly related to the role in the regulation of expression of key genes involved in chemotaxis and motility. This "dual role" is likely to influence the formation of biofilms by A. caulinodans and the host root colonization properties of this bacterium. |
| 文章类型 | Article |
| 资助机构 | NSFC-Shandong Joint Fund Key Projects [U1806206] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31570063, 31870020] ; Shandong Key Research and Development Program [2017GSF17129] ; Shandong Key Scientific and Technological Innovation Program [2017CXGC0303] ; Agricultural Scientific and Technological Innovation Project of Shandong Academy of Agricultural Sciences [+CXGC2016B10] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | BACILLUS-AMYLOLIQUEFACIENS ; ROOT COLONIZATION ; ORGANIC-ACIDS ; CHEMORECEPTORS ; CLONING ; SYSTEM ; GENE ; IDENTIFICATION ; NODULATION ; RESISTANCE |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences |
| WOS记录号 | WOS:000484540600009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24868 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Biol & Bioresource Utilizat, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; 2.Univ Chinese Acad Sci, Beijing, Peoples R China; 3.Shandong Huibang Bohai Agr Dev Ltd Co, Dongying, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, XL,Xie, ZH,Wang, YX,et al. A Dual Role of Amino Acids from Sesbania rostrata Seed Exudates in the Chemotaxis Response of Azorhizobium caulinodans ORS571[J]. MOLECULAR PLANT-MICROBE INTERACTIONS,2019,32(9):1134-1147. |
| APA | Liu, XL,Xie, ZH,Wang, YX,Sun, Y,Dang, XX,&Sun, HS.(2019).A Dual Role of Amino Acids from Sesbania rostrata Seed Exudates in the Chemotaxis Response of Azorhizobium caulinodans ORS571.MOLECULAR PLANT-MICROBE INTERACTIONS,32(9),1134-1147. |
| MLA | Liu, XL,et al."A Dual Role of Amino Acids from Sesbania rostrata Seed Exudates in the Chemotaxis Response of Azorhizobium caulinodans ORS571".MOLECULAR PLANT-MICROBE INTERACTIONS 32.9(2019):1134-1147. |
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| A Dual Role of Amino（1892KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
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