A sialic acid-binding lectin with bactericidal and opsonic activities from Ruditapes philippinarum

doi:10.1016/j.fsi.2019.08.074

	A sialic acid-binding lectin with bactericidal and opsonic activities from Ruditapes philippinarum
	Zhang, JN ; Zhang, YF ; Zhang, LB ; Wei, QY ; Liu, XL ; Yang, DL
发表期刊	FISH & SHELLFISH IMMUNOLOGY
ISSN	1050-4648
	2019-11
卷号	94 页码:72-80
关键词	Ruditapes philippinarum Sialic acid-binding lectin Pattern recognition receptor Immune recognition
研究领域	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
DOI	10.1016/j.fsi.2019.08.074
产权排序	[Zhang, Jianning; Zhang, Yifei; Liu, Xiaoli] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; [Liu, Xiaoli] Ludong Univ, Coastal Resources & Environm Team Blue Yellow Are, Yantai 264025, Peoples R China; [Zhang, Linbao] Chinese Acad Fisheries Sci, Guangdong Prov Key Lab Fishery Ecol & Environm, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China; [Yang, Dinglong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Wei, Qianyu; Yang, Dinglong] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
通讯作者	Liu, Xiaoli(lxlshz2006@163.com) ; Yang, Dinglong(dlyang@yic.ac.cn)
作者部门	中国科学院牟平海岸带环境综合试验站
英文摘要	In the present study, a sialic acid-binding lectin was cloned and characterized from Manila clam Ruditapes philippinarum (designed as RpSabl). The open reading frame of RpSabl encoded a polypepdde of 162 amino acids with a calculated molecular mass of 17.7 kDa. Analysis of the conserved domain suggested that RpSabl was a new member of the sialic acid-binding lectins family. In non-stimulated clams, RpSabl transcripts were constitutively expressed in all five tested tissues, especially in hepatopancreas. After Vibrio anguillarum challenge, the expression of RpSabl mRNA in hepatopancreas was significantly up-regulated at 3 h (3.8-fold, P < 0.05), 6 h (4.9-fold, P < 0.05), 12 h (12.3-fold, P < 0.01) and 24 h (9.7-fold, P < 0.01), while RpSabl transcripts in hemocytes was only significantly up-regulated at 6h (8.5-Fold, P < 0.01). MAI-mediated knockdown of RpSabl transcripts affected the survival rates of Manila clam against V. anguillarum, perhaps mainly due to the inhibited expression of antibacterial effectors (e.g. lysozyme and defensin). Moreover, recombinant protein of RpSabl (rRpSabl) possessed binding activities towards lipopolysaccharides (LPS), peptidoglycan (PGN) and glucan in vitro. Coinciding with the Pathogen-associated molecular patterns (PAMPs) binding assay, rRpSabl displayed broad bacterial-agglutination properties towards Vibrio harveyi, Vibrio splendidus, V. anguillarum, Enterobacter cloacae and Aeromonas hydrophila. Meanwhile, the phagocytosis and encapsulation ability of hemocytes could be significantly enhanced by rRpSabl incubation. All these results showed that RpSabl could function as a versatile molecule involved in the innate immune responses of R. philippinarum.
文章类型	Article
资助机构	Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA23050303] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41806196] ; Key Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [KFJ-STS-ZDTP-023] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2019BD022] ; Guangdong Provincial Key Laboratory of Fishery Ecology and Environment [LFE-2016-6] ; Youth Innovation Promotion Association of CAS [2016196]
收录类别	SCI
语种	英语
关键词[WOS]	C1Q-DOMAIN-CONTAINING PROTEIN ; ANTIBACTERIAL ACTIVITY ; IMMUNE ; COMPLEMENT ; C1Q ; RECOGNITION ; GENERATION ; MOLECULES ; PEPTIDE ; SABLS
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS记录号	WOS:000496892400009
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/24821
专题	支撑部门_中国科学院牟平海岸带环境综合试验站海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
作者单位	1.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; 2.Ludong Univ, Coastal Resources & Environm Team Blue Yellow Are, Yantai 264025, Peoples R China; 3.Chinese Acad Fisheries Sci, Guangdong Prov Key Lab Fishery Ecol & Environm, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China; 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; 5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Zhang, JN,Zhang, YF,Zhang, LB,et al. A sialic acid-binding lectin with bactericidal and opsonic activities from Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2019,94:72-80.
APA	Zhang, JN,Zhang, YF,Zhang, LB,Wei, QY,Liu, XL,&Yang, DL.(2019).A sialic acid-binding lectin with bactericidal and opsonic activities from Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,94,72-80.
MLA	Zhang, JN,et al."A sialic acid-binding lectin with bactericidal and opsonic activities from Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 94(2019):72-80.

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