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	Aeschynomene indica-Nodulating Rhizobia Lacking Nod Factor Synthesis Genes: Diversity and Evolution in Shandong Peninsula, China
	Zhang, ZP; Li, Y; Pan, XH; Shao, S; Liu, W; Wang, ET; Xie, ZH
发表期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN	0099-2240
	2019-11
卷号	85期号:22页码:e00782-19
关键词	Aeschynomene indica Rhizobium genetic diversity stem nodule photosynthetic genes molecular evolution evolution
研究领域	Biotechnology & Applied Microbiology ; Microbiology
DOI	10.1128/AEM.00782-19
产权排序	[Zhang, Zhenpeng; Li, Yan; Shao, Shuai; Liu, Wei; Xie, Zhihong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai, Peoples R China; [Zhang, Zhenpeng] UCAS, Beijing, Peoples R China; [Xie, Zhihong] Chinese Acad Sci, Ctr Ocean Mag Sci, Qingdao, Shandong, Peoples R China; [Shao, Shuai] Yantai Univ, Life Sci Coll, Yantai, Peoples R China; [Wang, En-Tao] Inst Politecn Nacl, Dept Microbiol, Escuela Nacl Ciencias Biol, Mexico City, DF, Mexico; [Pan, Xiaohan] China Agr Univ, Yantai Inst, Yantai, Peoples R China
通讯作者	Xie, Zhihong(zhxie@yic.ac.cn)
作者部门	海岸带生物学与生物资源保护实验室
英文摘要	Aeschynomene indica is a semiaquatic legume that forms both stem and root nodules with rhizobia. Some A. indica rhizobia (AIRs) have been reported to nodulate the host using a Nod factor-independent pathway and possess photosynthetic abilities. To investigate the diversity and community structure of AIRs in China, a total of 300 rhizobial isolates were acquired from the root and stem nodules of A. indica grown at 4 sites in Shandong Peninsula, China. Nineteen representative strains were selected according to their recA phylogeny. With further classification in comparison with reference strains, 10 Bradyrhizobium genospecies were defined based on the 16S rRNA gene phylogeny and multilocus sequence analysis (MLSA) of housekeeping genes (HKGs) recA, atpD, glnII, dnaK, gyrB, and rpoB. In addition, 6 genospecies were found only in China. No nodulation gene (nodA, nodB, nodC, or nodZ) was detected in the AIRs isolates by PCR amplification and Southern blotting. Phylogenetic analysis of nifH and the photosynthesis-related gene pufLM revealed their common origins. All representative strains formed root nodules, but only 9 representative strains for 4 genospecies formed stem nodules on A. indica, indicating that the stem nodulation process of A. indica is limited to some strains. The nucleotide diversity and recombination events of the HKGs, as well as nifH and pufLM genes, showed that mutation contributes more than recombination in evolution. The distribution of dominant AIR genospecies was mainly affected by available nitrogen, organic carbon, total nitrogen, and pH. Our study helps to characterize the diversity and evolution of AIRs. IMPORTANCE Aeschynomene indica rhizobia (AIRs) can form both root and stem nodules via Nod factor-independent processes, which distinguishes them from other rhizobia. This study systematically uncovered the diversity and community composition of A. indica rhizobia distributed in eastern China. Our results reclassified all the A. indica rhizobia across the world and represent a useful contribution to evaluating the diversity and distribution of the symbiont. The presence of novel genospecies specifically distributed in China enriched the A. indica rhizobia resources and provided insight into the geographic distribution of rhizobia. The phylogenetic relationship between nifH and pufLM of A. indica rhizobia across the world provides insight into the evolution of their nitrogen fixation and photosynthetic abilities.
文章类型	Article
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China [3160009, 31570063, 31870020] ; NSFC-Shandong Joint Fund Key Projects [U1806206] ; Key Deployment Project of the Chinese Academy of Sciences [KFZD-SW-112] ; Shandong Key Research and Development Program [2017GSF17129] ; Shandong Key Scientific and Technological Innovation Program [2017CXGC0303]
收录类别	SCI
语种	英语
关键词[WOS]	MULTILOCUS SEQUENCE-ANALYSIS ; NITROGEN-FIXING SYMBIONT ; STEM NODULATION ; SP NOV. ; PHOTOSYNTHETIC BRADYRHIZOBIA ; PHYLOGENETIC ANALYSIS ; EFFECTIVE NODULES ; INFECTION PROCESS ; GENOME SEQUENCE ; STRAIN ORS285
研究领域[WOS]	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000499128200001
引用统计	被引频次：6[WOS]   [WOS记录]     [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/24820
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai, Peoples R China; 2.UCAS, Beijing, Peoples R China; 3.Chinese Acad Sci, Ctr Ocean Mag Sci, Qingdao, Shandong, Peoples R China; 4.Yantai Univ, Life Sci Coll, Yantai, Peoples R China; 5.Inst Politecn Nacl, Dept Microbiol, Escuela Nacl Ciencias Biol, Mexico City, DF, Mexico; 6.China Agr Univ, Yantai Inst, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Zhang, ZP,Li, Y,Pan, XH,et al. Aeschynomene indica-Nodulating Rhizobia Lacking Nod Factor Synthesis Genes: Diversity and Evolution in Shandong Peninsula, China[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2019,85(22):e00782-19.
APA	Zhang, ZP.,Li, Y.,Pan, XH.,Shao, S.,Liu, W.,...&Xie, ZH.(2019).Aeschynomene indica-Nodulating Rhizobia Lacking Nod Factor Synthesis Genes: Diversity and Evolution in Shandong Peninsula, China.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,85(22),e00782-19.
MLA	Zhang, ZP,et al."Aeschynomene indica-Nodulating Rhizobia Lacking Nod Factor Synthesis Genes: Diversity and Evolution in Shandong Peninsula, China".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 85.22(2019):e00782-19.

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