| Specific genetic variation in two non-motile substrains of the model cyanobacterium Synechocystis sp. PCC 6803 | |
Chen Jun; Shi Wenjun; Li Wenjun; Chen Gao; Qin Song
| |
| 发表期刊 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| ISSN | 2096-5508 |
| 2018-11 | |
| 卷号 | 36期号:6 SI页码:2322-2332 |
| 关键词 | Synechocystis sp. PCC 6803 genome resequencing non-motile genetic background |
| 研究领域 | Marine & Freshwater Biology ; Oceanography |
| DOI | 10.1007/s00343-019-7291-1 |
| 产权排序 | [Chen Jun; Shi Wenjun; Li Wenjun; Qin Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Chen Jun; Shi Wenjun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Chen Gao] Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China |
| 作者部门 | 海岸带生物学与生物资源保护实验室 |
| 英文摘要 | Synechocystis sp. PCC 6803 is a model organism widely used in cyanobacterium biology and biotechnology. To know the genetic background of substrains of Synechocystis sp. PCC 6803 is important for further research and application. In this study, we reported the genome sequences of two non-motile wild-type substrains of Synechocystis sp. PCC 6803 using whole genome resequencing. 55/56 putative single nucleotide polymorphisms (SNPs) and 8/9 Indels (insertion and deletion) were identified. Among these, 47 SNPs were found in both the GT-AR and GT-CH strains, and 8 were unique to GT-AR and 9 were unique to GT-CH. All of these variations were annotated in metabolism pathway referred to KEGG database. Meanwhile, the deletion in slr0332 gene was detected in these two strains, which attributed to the non-motile phenotype of them and suggested that the insertion in spkA gene was not essential for non-motile phenotype. These resequencing data provide the genetic background information of these two strains and highlighted the microevolution over decades of laboratory cultivation. |
| 文章类型 | Article |
| 资助机构 | National Key Research and Development Program of China [2016YFF0202304] ; National Natural Science Foundation of China [41376139] ; Science and Technology Program of Yantai City [2016JHZB007] ; Natural Science Foundation of Shandong Province, China [ZR2016CM48] |
| 收录类别 | SCI |
| 语种 | 英语 |
| 关键词[WOS] | PURIFICATION ; MOTILITY ; SEQUENCE ; PCC-6803 ; GROWTH |
| 研究领域[WOS] | Limnology ; Oceanography |
| WOS记录号 | WOS:000454629000034 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.yic.ac.cn/handle/133337/24570 |
| 专题 | 海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Shandong Acad Agr Sci, Biotechnol Res Ctr, Jinan 250100, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen Jun,Shi Wenjun,Li Wenjun,et al. Specific genetic variation in two non-motile substrains of the model cyanobacterium Synechocystis sp. PCC 6803[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(6 SI):2322-2332. |
| APA | Chen Jun,Shi Wenjun,Li Wenjun,Chen Gao,&Qin Song.(2018).Specific genetic variation in two non-motile substrains of the model cyanobacterium Synechocystis sp. PCC 6803.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(6 SI),2322-2332. |
| MLA | Chen Jun,et al."Specific genetic variation in two non-motile substrains of the model cyanobacterium Synechocystis sp. PCC 6803".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.6 SI(2018):2322-2332. |
| 条目包含的文件 | ||||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Specific genetic var（279KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 请求全文 | |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论