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	Single Cell Analysis Linking Ribosomal (r)DNA and rRNA Copy Numbers to Cell Size and Growth Rate Provides Insights into Molecular Protistan Ecology
	Fu, R; Gong, J
发表期刊	JOURNAL OF EUKARYOTIC MICROBIOLOGY
ISSN	1066-5234
	2017-11
卷号	64期号:6页码:885-896
关键词	Activity body Body size copy number variation experimental warming nutrient cycling ribosome content
研究领域	Microbiology
DOI	10.1111/jeu.12425
产权排序	[Fu, Rao; Gong, Jun] Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China; [Fu, Rao; Gong, Jun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Gong, Jun] Sun Yat Sen Univ, Sch Marine Sci, Lab Microbial Ecol & Matter Cycles, Zhuhai 519082, Peoples R China
作者部门	海岸带生物学与生物资源保护实验室
英文摘要	Ribosomal (r)RNA and rDNA have been golden molecular markers in microbial ecology. However, it remains poorly understood how ribotype copy number (CN)-based characteristics are linked with diversity, abundance, and activity of protist populations and communities observed at organismal levels. Here, we applied a single-cell approach to quantify ribotype CNs in two ciliate species reared at different temperatures. We found that in actively growing cells, the per-cell rDNA and rRNA CNs scaled with cell volume (CV) to 0.44 and 0.58 powers, respectively. The modeled rDNA and rRNA concentrations thus appear to be much higher in smaller than in larger cells. The observed rRNA:rDNA ratio scaled with CV0.14. The maximum growth rate could be well predicted by a combination of per-cell ribotype CN and temperature. Our empirical data and modeling on single-cell ribotype scaling are in agreement with both the metabolic theory of ecology and the growth rate hypothesis, providing a quantitative framework for linking cellular rDNA and rRNA CNs with body size, growth (activity), and biomass stoichiometry. This study also demonstrates that the expression rate of rRNA genes is constrained by cell size, and favors biomass rather than abundance-based interpretation of quantitative ribotype data in population and community ecology of protists.
文章类型	Article
资助机构	National Natural Science Foundation of China [31572255, 41522604] ; Strategic Priority Research Program of CAS [XDA11020702] ; Open Fund of Qingdao National Laboratory for Marine Science and Technology [QNLM2016ORP0306] ; Key Research Program of Frontier Sciences of CAS [QYZDB-SSW-DQC013]
收录类别	SCI
语种	英语
关键词[WOS]	Activity ; body ; Body size ; copy number variation ; experimental warming ; nutrient cycling ; ribosome content
研究领域[WOS]	Microbiology
WOS记录号	WOS:000415179600014
引用统计	被引频次：41[WOS]   [WOS记录]     [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/24351
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Sun Yat Sen Univ, Sch Marine Sci, Lab Microbial Ecol & Matter Cycles, Zhuhai 519082, Peoples R China
推荐引用方式 GB/T 7714	Fu, R,Gong, J. Single Cell Analysis Linking Ribosomal (r)DNA and rRNA Copy Numbers to Cell Size and Growth Rate Provides Insights into Molecular Protistan Ecology[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2017,64(6):885-896.
APA	Fu, R,&Gong, J.(2017).Single Cell Analysis Linking Ribosomal (r)DNA and rRNA Copy Numbers to Cell Size and Growth Rate Provides Insights into Molecular Protistan Ecology.JOURNAL OF EUKARYOTIC MICROBIOLOGY,64(6),885-896.
MLA	Fu, R,et al."Single Cell Analysis Linking Ribosomal (r)DNA and rRNA Copy Numbers to Cell Size and Growth Rate Provides Insights into Molecular Protistan Ecology".JOURNAL OF EUKARYOTIC MICROBIOLOGY 64.6(2017):885-896.

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