YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
A mitochondrial-targeting near-infrared fluorescent probe for bioimaging and evaluating endogenous superoxide anion changes during ischemia/reperfusion injury
Han, Xiaoyue1,4; Wang, Rui1; Song, Xinyu2,3; Yu, Fabiao1,3; Lv, Changjun2,3; Chen, Lingxin1,3
2018-02-01
Source PublicationBIOMATERIALS
ISSN0142-9612
Volume156Pages:134-146
AbstractThe outburst of superoxide anion (O-2(center dot-)) in mitochondrial during ischemia/reperfusion (I/R) process will cause a series of oxidative damage including polarity loss of mitochondrial membrane potential, overload of secondary cellular calcium, and cascade apoptosis. To monitor the O-2(center dot-) level fluctuations as well as to evaluate the relationship between O-2(center dot-) concentration and the degree of cell apoptosis during I/R process, we propose a ratiometric near-infrared mitochondrial targeting fluorescent probe Mito-Cy-Tfs for the detection of level changes of O-2(center dot-) in cells and in vivo. The probe Mito-Cy-Tfs is composed of three moieties: near-infrared heptamethine cyanine as fluorescence signal transducer, trifluoromethanesulfonamide as fluorescence modulator, and lipophilic triphenylphosphonium cation as mitochondrial guider. The probe can well locate in mitochondria and respond the concentration changes of endogenous O-2(center dot-) selectively and sensitively. The probe has been successfully utilized to image the endogenous O-2(center dot-) fluctuations in four kinds of cell I/R models (glucose deprivation/reperfusion, serum deprivation/reperfusion, oxygen deprivation/reperfusion and glucose-serum-oxygen deprivation/reperfusion). The probe also exhibits deep tissue penetration for real-time imaging of O-2(center dot-) concentration in liver of I/R mice model. We confirm that the adoption of ischemic preconditioning (IPC) and postconditioning (IPTC) can protect liver from I/R injury. The probe can be employed to accurately indicate and evaluate the mutual relationship between the levels of O-2(center dot-) and the degrees of organ damage during I/R, IPC and IPTC processes. The above applications make our new probe a potential candidate for the clinical surgery assessment. (C) 2017 Elsevier Ltd. All rights reserved.
KeywordNear-infrared Fluorescent Probes Ratiometric Bioimaging Superoxide Anion Mitochondrial Localization Ischemia/reperfusion Injury
DOI10.1016/j.biomaterials.2017.11.039
Funding OrganizationNational Nature Science Foundation of China(21775162, 21405172, 41776110, 21575159) ; Youth Innovation Promotion Association, CAS(2015170) ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS(KF2016-22) ; Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, Qingdao University of Science and Technology(SATM201705) ; Chinese Academy of Sciences(YZ201662)
Indexed BySCI
WOS KeywordIN-VIVO ; REACTIVE OXYGEN ; FREE-RADICALS ; LIVING CELLS ; LIVE CELLS ; HYDROGEN POLYSULFIDES ; DESIGN ; DEATH ; APOPTOSIS ; ANTIOXIDANTS
SubtypeArticle
Language英语
WOS Research AreaEngineering ; Materials Science
WOS SubjectEngineering, Biomedical ; Materials Science, Biomaterials
WOS IDWOS:000419539100011
PublisherELSEVIER SCI LTD
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/24051
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorYu, Fabiao; Chen, Lingxin
Affiliation1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
2.Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China
3.Binzhou Med Univ, Inst Mol Med, Med Res Ctr, Yantai 264003, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
First Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Corresponding Author AffilicationYantai Institute of Coastal Zone Research, Chinese Academy of Sciences
Recommended Citation
GB/T 7714
Han, Xiaoyue,Wang, Rui,Song, Xinyu,et al. A mitochondrial-targeting near-infrared fluorescent probe for bioimaging and evaluating endogenous superoxide anion changes during ischemia/reperfusion injury[J]. BIOMATERIALS,2018,156:134-146.
APA Han, Xiaoyue,Wang, Rui,Song, Xinyu,Yu, Fabiao,Lv, Changjun,&Chen, Lingxin.(2018).A mitochondrial-targeting near-infrared fluorescent probe for bioimaging and evaluating endogenous superoxide anion changes during ischemia/reperfusion injury.BIOMATERIALS,156,134-146.
MLA Han, Xiaoyue,et al."A mitochondrial-targeting near-infrared fluorescent probe for bioimaging and evaluating endogenous superoxide anion changes during ischemia/reperfusion injury".BIOMATERIALS 156(2018):134-146.
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