A mitochondrial-targeting near-infrared fluorescent probe for bioimaging and evaluating endogenous superoxide anion changes during ischemia/reperfusion injury
Han, Xiaoyue1,4; Wang, Rui1; Song, Xinyu2,3; Yu, Fabiao1,3; Lv, Changjun2,3; Chen, Lingxin1,3
2018-02-01
发表期刊BIOMATERIALS
ISSN0142-9612
卷号156页码:134-146
摘要The outburst of superoxide anion (O-2(center dot-)) in mitochondrial during ischemia/reperfusion (I/R) process will cause a series of oxidative damage including polarity loss of mitochondrial membrane potential, overload of secondary cellular calcium, and cascade apoptosis. To monitor the O-2(center dot-) level fluctuations as well as to evaluate the relationship between O-2(center dot-) concentration and the degree of cell apoptosis during I/R process, we propose a ratiometric near-infrared mitochondrial targeting fluorescent probe Mito-Cy-Tfs for the detection of level changes of O-2(center dot-) in cells and in vivo. The probe Mito-Cy-Tfs is composed of three moieties: near-infrared heptamethine cyanine as fluorescence signal transducer, trifluoromethanesulfonamide as fluorescence modulator, and lipophilic triphenylphosphonium cation as mitochondrial guider. The probe can well locate in mitochondria and respond the concentration changes of endogenous O-2(center dot-) selectively and sensitively. The probe has been successfully utilized to image the endogenous O-2(center dot-) fluctuations in four kinds of cell I/R models (glucose deprivation/reperfusion, serum deprivation/reperfusion, oxygen deprivation/reperfusion and glucose-serum-oxygen deprivation/reperfusion). The probe also exhibits deep tissue penetration for real-time imaging of O-2(center dot-) concentration in liver of I/R mice model. We confirm that the adoption of ischemic preconditioning (IPC) and postconditioning (IPTC) can protect liver from I/R injury. The probe can be employed to accurately indicate and evaluate the mutual relationship between the levels of O-2(center dot-) and the degrees of organ damage during I/R, IPC and IPTC processes. The above applications make our new probe a potential candidate for the clinical surgery assessment. (C) 2017 Elsevier Ltd. All rights reserved.
关键词Near-infrared Fluorescent Probes Ratiometric Bioimaging Superoxide Anion Mitochondrial Localization Ischemia/reperfusion Injury
DOI10.1016/j.biomaterials.2017.11.039
项目资助者National Nature Science Foundation of China(21775162, 21405172, 41776110, 21575159) ; Youth Innovation Promotion Association, CAS(2015170) ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS(KF2016-22) ; Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, Qingdao University of Science and Technology(SATM201705) ; Chinese Academy of Sciences(YZ201662)
收录类别SCI
关键词[WOS]IN-VIVO ; REACTIVE OXYGEN ; FREE-RADICALS ; LIVING CELLS ; LIVE CELLS ; HYDROGEN POLYSULFIDES ; DESIGN ; DEATH ; APOPTOSIS ; ANTIOXIDANTS
文章类型Article
语种英语
WOS研究方向Engineering ; Materials Science
WOS类目Engineering, Biomedical ; Materials Science, Biomaterials
WOS记录号WOS:000419539100011
出版者ELSEVIER SCI LTD
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/24051
专题中科院海岸带环境过程与生态修复重点实验室
通讯作者Yu, Fabiao; Chen, Lingxin
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
2.Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China
3.Binzhou Med Univ, Inst Mol Med, Med Res Ctr, Yantai 264003, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
通讯作者单位中国科学院烟台海岸带研究所
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Han, Xiaoyue,Wang, Rui,Song, Xinyu,et al. A mitochondrial-targeting near-infrared fluorescent probe for bioimaging and evaluating endogenous superoxide anion changes during ischemia/reperfusion injury[J]. BIOMATERIALS,2018,156:134-146.
APA Han, Xiaoyue,Wang, Rui,Song, Xinyu,Yu, Fabiao,Lv, Changjun,&Chen, Lingxin.(2018).A mitochondrial-targeting near-infrared fluorescent probe for bioimaging and evaluating endogenous superoxide anion changes during ischemia/reperfusion injury.BIOMATERIALS,156,134-146.
MLA Han, Xiaoyue,et al."A mitochondrial-targeting near-infrared fluorescent probe for bioimaging and evaluating endogenous superoxide anion changes during ischemia/reperfusion injury".BIOMATERIALS 156(2018):134-146.
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