A unique off-on near-infrared cyanine-based probe for imaging of endogenous alkaline phosphatase activity in cells and in vivo

doi:10.1016/j.snb.2018.03.078

YIC-IR

	A unique off-on near-infrared cyanine-based probe for imaging of endogenous alkaline phosphatase activity in cells and in vivo
	Gao, Zhiwei 1,2; Sun, Jingya 1; Gao, Min 2,3; Yu, Fabiao 1,2; Chen, Lingxin 1,2; Chen, Qingguo 1
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
ISSN	0925-4005
	2018-07-15
卷号	265 页码:565-574
关键词	Alkaline phosphatase Fluorescent probes Near-infrared fluorescence In vivo imaging
DOI	10.1016/j.snb.2018.03.078
英文摘要	Alkaline phosphatase (ALP) is a class of enzymes that are widely found in various tissues of the human body. It plays important roles in regulating diverse cellular functions. The aberrant levels of serum ALP are implicated in diseases. The development of sensitive and accurate detection tool for evaluating the level changes of ALP in living organisms will be very helpful in the fields of biochemistry, cytology and clinical medicine. Herein, we develop a near-infrared probe (QcyP) for the specific detection of different ALP levels both in different cell lines and in tumor-bearing mice models. The probe is composed of two moieties: a unique heptamethine cyanine fluorophore and a phosphate monoester. ALP can trigger an off-on fluorescence switch via an enzyme-catalyzed cleavage of the phosphate group. The fluorescence regulation mechanism is based on the rearrangement of the conjugated pi-electron system. The probe exhibits high selectivity and good sensitivity towards ALP. The results demonstrate that the probe QcyP not only can be applied to discriminate the levels of ALP in different cell lines, but also can distinguish the ALP level changes between hepatic tumor mice model and normal mice. (C) 2018 Elsevier B.V. All rights reserved.
资助机构	National Nature Science Foundation of China ; program of Youth Innovation Promotion Association, CAS ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CAS ; Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, Qingdao University of Science and Technology
语种	英语
关键词[WOS]	RATIOMETRIC FLUORESCENT-PROBE ; LIVING CELLS ; QUANTUM DOTS ; CARBON DOTS ; ASSAY ; NITROREDUCTASE ; NANOPARTICLES ; PLATFORMS ; PARADIGM ; BIOLOGY
研究领域[WOS]	Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:000430232500068
引用统计
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/23903
通讯作者	Yu, Fabiao; Chen, Lingxin; Chen, Qingguo
作者单位	1.Zhejiang Ocean Univ, Coll Marine Sci & Technol, Zhoushan 316004, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Gao, Zhiwei,Sun, Jingya,Gao, Min,et al. A unique off-on near-infrared cyanine-based probe for imaging of endogenous alkaline phosphatase activity in cells and in vivo[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,265:565-574.
APA	Gao, Zhiwei,Sun, Jingya,Gao, Min,Yu, Fabiao,Chen, Lingxin,&Chen, Qingguo.(2018).A unique off-on near-infrared cyanine-based probe for imaging of endogenous alkaline phosphatase activity in cells and in vivo.SENSORS AND ACTUATORS B-CHEMICAL,265,565-574.
MLA	Gao, Zhiwei,et al."A unique off-on near-infrared cyanine-based probe for imaging of endogenous alkaline phosphatase activity in cells and in vivo".SENSORS AND ACTUATORS B-CHEMICAL 265(2018):565-574.