Institutional Repository of Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences (KLCEP)
| Wide-Acidity-Range pH Fluorescence Probes for Evaluation of Acidification in Mitochondria and Digestive Tract Mucosa | |
Chen, Guang1,2,3,4; Fu, Qiang1,2; Yu, Fabiao1,2,3; Ren, Rui4; Liu, Yuxia1,2; Cao, Ziping1,2; Li, Guoliang1,2; Zhao, Xianen1,2; Chen, Lingxin1,2,3 ; Wang, Hua1,2; You, Jinmao1,2,3; Yu, Fabiao(Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal, Qufu 273165, Peoples R China); Chen, Lingxin(Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal, Qufu 273165, Peoples R China); You, Jinmao(Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal, Qufu 273165, Peoples R China)
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| 2017-08-15 | |
| Source Publication | ANALYTICAL CHEMISTRY
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| ISSN | 0003-2700 |
| Volume | 89Issue:16Pages:8509-8516 |
| Contribution Rank | 第3完成单位 |
| Abstract | The cells control their pH change in a very accurate range. pH plays important roles in cell autophagy and apoptosis. Previous evidence implies that the internal milieu of a tumor is acidified. Although the acidification in cells is investigated, the biological effects from multiple stimulating factors under the complex intracellular environment have not been thoroughly elaborated yet. Currently, there are few pH probes that perform in a wide acidity range, and a probe that is capable of measuring a wide pH range needs to be developed. Herein, we report two new fluorescent probes (BHNBD and CM-BHNBD) for the detection of mitochondrial and intramucosal acidification. The two probes respond to pH via an H+-driven TICT (twist intramolecular charge transfer) mechanism, and they can linearly report pH within a wide pH range: 7.00-2.00 following similar to 148-fold fluorescence increase. The two probes also possess excellent membrane permeability, good photostability, and negligible cytotoxicity. The probes are successfully applied for quantifying the acidification in HeLa cells under the simultaneous stimulation of nutrient deprivation and oxidative stress. Our results demonstrate that the mitochondrial pH is in a dynamic fluctuating state during the acidification process, which suggests a potential cross-talk effect between cell autophagy and apoptosis. We also use the probes for quantifying the intramucosal pH variation in stomach and esophagus via manipulating cellular proton pump. The development of our probes is potentially expected to be used to monitor the intracellular/intramucosal acidification for biomedical research. |
| DOI | 10.1021/acs.analchem.7b02164 |
| Funding Organization | National Natural Science Foundation of China(21475074, 21403123, 21375075, 31301595, 21402106, 21475075, 21275089, 21405093, 21405094, 81303179, 21305076) ; Shandong Province Key Laboratory of Detection Technology for Tumor Markers(KLDTTM2015-6, KLDTTM2015-9) ; Taishan Scholar Foundation of Shandong Province |
| URL | 查看原文 |
| Indexed By | SCI |
| WOS Keyword | OXIDATIVE STRESS ; LIVING CELLS ; DESIGN STRATEGIES ; HYDROGEN-PEROXIDE ; MECHANISM ; APOPTOSIS ; AUTOPHAGY ; MITOPHAGY ; CHEMOSELECTIVITY ; HETEROGENEITY |
| Subtype | Article |
| Language | 英语 |
| WOS Research Area | Chemistry |
| WOS ID | WOS:000407988600047 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.yic.ac.cn/handle/133337/23423 |
| Collection | 中科院海岸带环境过程与生态修复重点实验室 |
| Corresponding Author | Yu, Fabiao; Chen, Lingxin; You, Jinmao; Yu, Fabiao(Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal, Qufu 273165, Peoples R China); Chen, Lingxin(Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal, Qufu 273165, Peoples R China); You, Jinmao(Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal, Qufu 273165, Peoples R China) |
| Affiliation | 1.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal, Qufu 273165, Peoples R China 2.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 4.Linyi Univ, Coll Chem & Chem Engn, Shandong Prov Key Lab Detect Technol Tumor Makers, Linyi 276005, Peoples R China |
| First Author Affilication | Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences |
| Corresponding Author Affilication | Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Chen, Guang,Fu, Qiang,Yu, Fabiao,et al. Wide-Acidity-Range pH Fluorescence Probes for Evaluation of Acidification in Mitochondria and Digestive Tract Mucosa[J]. ANALYTICAL CHEMISTRY,2017,89(16):8509-8516. |
| APA | Chen, Guang.,Fu, Qiang.,Yu, Fabiao.,Ren, Rui.,Liu, Yuxia.,...&You, Jinmao.(2017).Wide-Acidity-Range pH Fluorescence Probes for Evaluation of Acidification in Mitochondria and Digestive Tract Mucosa.ANALYTICAL CHEMISTRY,89(16),8509-8516. |
| MLA | Chen, Guang,et al."Wide-Acidity-Range pH Fluorescence Probes for Evaluation of Acidification in Mitochondria and Digestive Tract Mucosa".ANALYTICAL CHEMISTRY 89.16(2017):8509-8516. |
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