The invention relates to the field of insecticides, bacteriostats and tumor cell inhibitors, and in particular relates to a drop diterpenoid type compound from seaweed endophytes as well as a preparation method and application of the drop diterpenoid type compound. The drop diterpenoid type compound has a structural formula shown by a formula (I). The preparation method of the compound comprises the steps of inoculating Aspergillus wentii NA3 to a fungus liquid culture medium for fermentation culture, and purifying a fermentation product to obtain the drop diterpenoid type compound shown by the formula (I). An insecticidal activity test shows that the median lethal concentration of the drop diterpenoid type compound obtained by the preparation method disclosed by the invention is 1.81 micrograms per milliliter, and meanwhile, the compound has activities of inhibiting bacteria and tumor cells.
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