The invention relates to a chymotrypsin inhibitor from urechis unicincts as well as an application of a gene and a recombinant protein of the chymotrypsin inhibitor, belonging to the biomedical field. The protein of the chymotrypsin inhibitor has an amino acid sequence as shown in SEQ ID No.1; the coded urechis unicincts chymotrypsin inhibitor UCI01 is single strand polypeptide, has molecular weight of about 7516Da and an isoelectric point of 4.38, contains 67 amino acid residues and does not contain a disulfide bond and an obvious signal peptide area. The gene has a base sequence as shown in SEQ ID No.2 in a sequence table, the overall length of the cDNA sequence is 697bp, the cDNA sequence contains a 80bp 5' non-translated region, a 413bp 3' non-translated region and a polyA tail, and the length of an open coding frame is 201bp. A prokaryotic expression technology is utilized to perform recombinant expression on the gene, so that the obtained recombinant protein has activity of the chymotrypsin inhibitor, and IC50 of the recombinant protein is 53nM. The recombinant protein has relatively good pH stability and a certain temperature stability, and has antibacterial effects on staphylococcus aureus.
; 杨少丽
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