YIC-IR  > 中科院海岸带环境过程与生态修复重点实验室
样品前处理方式对沉积物酶水解有机磷的影响
Alternative TitleEffect of sample pretreatment on enzymatic hydrolysis of organic phosphorus in sediment
张友; 吕迎春; 高丽; 刘书花; 徐刚; 王安东; 赵亚杰
2017-02-15
Source Publication环境化学
ISSN0254-6108
Volume36Issue:1Pages:107-113
Contribution Rank(1)中国科学院烟台海岸带研究所;(2)中国科学院大学;(3)烟台大学;(4)山东省分析测试中心;(5)山东省黄河三角洲国家级自然保护区管理局;
Abstract磷酸酶水解技术是研究沉积物有机磷形态和生物有效性的有力工具.沉积物样品处理过程对酶水解有机磷的影响尚不清楚.为此,以天鹅湖表层沉积物为研究对象,研究了干燥方式、提取剂、提取比例、提取时间条件对沉积物酶水解有机磷的影响特征,以寻求最佳的酶水解技术的实验条件.研究结果表明,自然风干处理引发了有机磷水解,降低了磷酸二酯磷(Diester-P)含量.而冻干处理更真实地保存了沉积物有机磷形态特征,比较合适有机磷分析.尽管HCl预提取显著增大了有机磷的提取比例,但是存在Diester-P水解风险,因此Na OH/EDTA一步提取更适合有机磷的测定.较长的提取时间和较大固液比同样也显著增大了有机磷的提取效率,但是同样存在Diester-P水解风险.研究显示Diester-P极不稳定,易受实验条件的影响,在实际操作中需要特别注意.总之沉积物样品采用冷冻干燥处理,固液比1∶30,采用0.25 mol·L-1Na OH+50 mmol·L-1EDTA提8 h更适合沉积物酶水解有机磷的分析. 
Other AbstractEnzymatic hydrolysis is a powerful tool to study the speciation and bioavailability ofsediment organic phosphorus ( P) £® However£¬the effects of the sample treatment on the enzymatichydrolysis need to be fully understood£® In order to investigate the optimal condition for enzymatichydrolysis£¬the surface sediments of Swan Lake were collected to study the effects of samplepreparation procedures£¬including sample pretreatment£¬extraction solution£¬extraction time£¬andratio of sample to extraction efficiency on enzymatic hydrolysis£® The results showed that air-dryingcaused the hydrolysis of diester-P in sediments£® Freeze drying preserved the characteristics of organicphosphorus£¬and is recommended for the study of organic phosphorus in sediments£® Although the recovery of P was significantly increased by HCl pre-extraction,there is also a risk of diester-Phydrolysis at the same time. As a result,one step extraction of NaOH/EDTA is recommended forenzymatic hydrolysis of organic phosphorus in sediment. In addition,longer extraction time andhigher extraction ratio ( S ( sediment ) /V ( volume ) ) tend to increased the risk of diester-Phydrolysis although they increased the recovery of P from sediment. Our results suggest that diester-Pin sediment is extremely unstable and vulnerable to extraction conditions. Thus it deserves specialcaution with sample analysis and data interpretation. In conclusion,the freeze dried sediments wereextracted with 0.25 mol·L-1 NaOH+50 mmol·L-1 EDTA at 8 h and sediment /solution ratio of 1∶30which were recommended for enzymatic hydrolysis of organic phosphorus in sediments.
Keyword沉积物 磷酸酶 有机磷 提取条件
Department中科院海岸带环境过程与生态修复重点实验室
Funding Organization国家自然科学基金(41573120,41273130) ; 山东省科技攻关计划(2014GGH02004)资助~~
Indexed ByCSCD
Language中文
CSCD IDCSCD:5946176
Citation statistics
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/22081
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding Author徐刚
Affiliation1.中国科学院烟台海岸带研究所
2.中国科学院大学
3.烟台大学
4.山东省分析测试中心
5.山东省黄河三角洲国家级自然保护区管理局
Recommended Citation
GB/T 7714
张友,吕迎春,高丽,等. 样品前处理方式对沉积物酶水解有机磷的影响[J]. 环境化学,2017,36(1):107-113.
APA 张友.,吕迎春.,高丽.,刘书花.,徐刚.,...&赵亚杰.(2017).样品前处理方式对沉积物酶水解有机磷的影响.环境化学,36(1),107-113.
MLA 张友,et al."样品前处理方式对沉积物酶水解有机磷的影响".环境化学 36.1(2017):107-113.
Files in This Item: Download All
File Name/Size DocType Version Access License
样品前处理方式对沉积物酶水解有机磷的影响(244KB)期刊论文作者接受稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[张友]'s Articles
[吕迎春]'s Articles
[高丽]'s Articles
Baidu academic
Similar articles in Baidu academic
[张友]'s Articles
[吕迎春]'s Articles
[高丽]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[张友]'s Articles
[吕迎春]'s Articles
[高丽]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 样品前处理方式对沉积物酶水解有机磷的影响.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.