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In situ quantification and evaluation of ClO-/H2S homeostasis in inflammatory gastric tissue by applying a rationally designed dual-response fluorescence probe featuring a novel H+-activated mechanism
Fu, Qiang1,2; Chen, Guang1,3; Liu, Yuxia1; Cao, Ziping1; Zhao, Xianen1; Li, Guoliang1; Yu, Fabiao3; Chen, Lingxin3; Wang, Hua1; You, Jinmao1,2; Chen, G
2017-05-07
Source PublicationANALYST
ISSN0003-2654
Volume142Issue:9Pages:1619-1627
Contribution Rank[Fu, Qiang; Chen, Guang; Liu, Yuxia; Cao, Ziping; Zhao, Xianen; Li, Guoliang; Wang, Hua; You, Jinmao] Qufu Normal Univ, Key Lab Life Organ Anal, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China; [Fu, Qiang; You, Jinmao] Qinghai Normal Univ, Xining, Peoples R China; [Chen, Guang; Yu, Fabiao; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
AbstractHomeostasis of ClO-/H2S plays a crucial role in the damage and repair of gastric tissue, but has rarely been investigated due to the challenge of in situ analysis in the highly acidic gastric environment. Herein, we designed a new H+-activated optical mechanism, involving controllable photoinduced electron transfer (PET) and switch of electron push-pull (SEPP), to develop the simple yet multifunctional probe (Z)-4( 2-benzylidenehydrazinyl)-7-nitrobenzo[c][1,2,5] oxadiazole (BNBD). First, the BNBD probe (Off) was protonated by the highly acidic media to trigger strong fluorescence (On). Then, the analytes ClO- and H2S reacted with the protonated BNBD, leading to ultrasensitive (ClO-: 2.7 nM and H2S: 6.9 nM) fluorescence quenching via the rapid oxidation of CvN (50 s) and nitro reduction (10 s), respectively. With the logical discrimination by absorbance/colour (ClO-: 300 nm/colorless and H2S: 400 nm/orange), a strategy for the in situ quantification of ClO-/H2S in gastric mucosa and juice was developed. For the first time, the in situ quantitative monitoring of endogenous H2S and ClO-/H2S homeostasis as well as the pathologic manifestation in gastric mucosa were realized, thus overcoming the challenge of ClO-/H2S analysis under highly acidic conditions and enabling the in situ tissue quantification of ClO-/H2S. In combination with the assessment of mucosal damage, this study confirms the injurious/rehabilitative effects of ClO-/H2S on gastric mucosa (at 50-90 mu m depth), which may facilitate the auxiliary diagnosis of stomach diseases induced by oxidative stress.
KeywordEndogenous Hydrogen-sulfide Living Cells Hypochlorous Acid Biological-systems Oxidative Stress Redox Cycle Se-bodipy Polysulfides Mitochondria Damage
Department中科院海岸带环境过程与生态修复重点实验室
Subject AreaChemistry
DOI10.1039/c7an00244k
Funding OrganizationNational Natural Science Foundation of China(21475074 ; Taishan Scholar Foundation of Shandong Province, P. R. China ; Open Funds of the Shandong Province Key Laboratory of Detection Technology for Tumor Markers(KLDTTM2015-6) ; Province Higher Educational Science and Technology Program(J14LC17) ; Opening Foundation of Shandong Provincial Key Laboratory of Detection Technology for Tumor Makers(KLDTTM2015-9) ; China Postdoctoral Science Foundation(2016 M600531) ; 21403123 ; 21375075 ; 31301595 ; 21402106 ; 21475075 ; 21275089 ; 21405093 ; 21405094 ; 81303179 ; 21305076)
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Indexed BySCI
WOS KeywordENDOGENOUS HYDROGEN-SULFIDE ; LIVING CELLS ; HYPOCHLOROUS ACID ; BIOLOGICAL-SYSTEMS ; OXIDATIVE STRESS ; REDOX CYCLE ; SE-BODIPY ; POLYSULFIDES ; MITOCHONDRIA ; DAMAGE
SubtypeArticle
Language英语
WOS Research AreaChemistry
WOS IDWOS:000400688500025
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.yic.ac.cn/handle/133337/22049
Collection中科院海岸带环境过程与生态修复重点实验室
Corresponding AuthorChen, G
Affiliation1.Qufu Normal Univ, Key Lab Life Organ Anal, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Shandong, Peoples R China
2.Qinghai Normal Univ, Xining, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
Recommended Citation
GB/T 7714
Fu, Qiang,Chen, Guang,Liu, Yuxia,et al. In situ quantification and evaluation of ClO-/H2S homeostasis in inflammatory gastric tissue by applying a rationally designed dual-response fluorescence probe featuring a novel H+-activated mechanism[J]. ANALYST,2017,142(9):1619-1627.
APA Fu, Qiang.,Chen, Guang.,Liu, Yuxia.,Cao, Ziping.,Zhao, Xianen.,...&Chen, G.(2017).In situ quantification and evaluation of ClO-/H2S homeostasis in inflammatory gastric tissue by applying a rationally designed dual-response fluorescence probe featuring a novel H+-activated mechanism.ANALYST,142(9),1619-1627.
MLA Fu, Qiang,et al."In situ quantification and evaluation of ClO-/H2S homeostasis in inflammatory gastric tissue by applying a rationally designed dual-response fluorescence probe featuring a novel H+-activated mechanism".ANALYST 142.9(2017):1619-1627.
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