Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor

doi:10.1021/acs.analchem.6b03536

	Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor
	Wang, Xiaokun ; Choi, Namhyun ; Cheng, Ziyi ; Ko, Juhui ; Chen, Lingxin; Choo, Jaebum ; Choo, J (reprint author), Hanyang Univ, Dept Bionano Engn, Ansan 426791, South Korea.
发表期刊	ANALYTICAL CHEMISTRY
ISSN	0003-2700
	2017-01-17
卷号	89 期号:2 页码:1163-1169
DOI	10.1021/acs.analchem.6b03536
产权排序	[Wang, Xiaokun; Choi, Namhyun; Cheng, Ziyi; Ko, Juhui; Choo, Jaebum] Hanyang Univ, Dept Bionano Engn, Ansan 426791, South Korea; [Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
作者部门	中科院海岸带环境过程与生态修复重点实验室
英文摘要	A new class of surface-enhanced Raman scattering (SERS)-based lateral flow assay (LFA) biosensor has been developed for the simultaneous detection of dual DNA markers. The LFA strip in this sensor was composed of two test lines and one control line. SERS nano tags labeled with detection DNA probes were used for quantitative evaluation of dual DNA markers with high sensitivity. Target DNA, associated with Kaposis sarcoma-associated herpesvirus (KSHV) and bacillary angiomatosis (BA), were tested to validate the detection capability of this SERS-based LFA strip. Characteristic peak intensities of SERS nano tags on two test lines were used for quantitative evaluations of KSHV and BA. The limits of detection for KSHV and BA, determined from our SERS-based LFA sensing platform, were estimated to be 0.043 and 0.074 pM, respectively. These values indicate approximately 10000 times higher sensitivity than previously reported values using the aggregation-based colorimetric method. We believe that this is the first report of simultaneous detection of two different DNA mixtures using a SERS-based LFA platform. This novel detection technique is also a promising multiplex DNA sensing platform for early disease diagnosis.
文章类型	Article
资助机构	Korea Health Industry Development Institute (KHIDI) - Ministry of Health and Welfare, Korea(HI16C2129) ; National Natural Science Foundation of China(21575159 ; 21275158)
收录类别	SCI
语种	英语
关键词[WOS]	MULTICOLOR QUANTUM DOTS ; KAPOSIS-SARCOMA ; BACILLARY ANGIOMATOSIS ; QUANTITATIVE DETECTION ; TEST STRIP ; IMMUNOASSAY ; GOLD ; POINT ; AIDS ; NANOPARTICLES
研究领域[WOS]	Chemistry
WOS记录号	WOS:000392458100021
引用统计	被引频次：188[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/20230
专题	中国科学院海岸带环境过程与生态修复重点实验室
通讯作者	Choo, J (reprint author), Hanyang Univ, Dept Bionano Engn, Ansan 426791, South Korea.
作者单位	1.Hanyang Univ, Dept Bionano Engn, Ansan 426791, South Korea 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xiaokun,Choi, Namhyun,Cheng, Ziyi,et al. Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor[J]. ANALYTICAL CHEMISTRY,2017,89(2):1163-1169.
APA	Wang, Xiaokun.,Choi, Namhyun.,Cheng, Ziyi.,Ko, Juhui.,Chen, Lingxin.,...&Choo, J .(2017).Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor.ANALYTICAL CHEMISTRY,89(2),1163-1169.
MLA	Wang, Xiaokun,et al."Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor".ANALYTICAL CHEMISTRY 89.2(2017):1163-1169.

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