Isolation and characterization of astaxanthin-hyperproducing mutants of Haematococcus pluvialis (Chlorophyceae) produced by dielectric barrier discharge plasma

doi:10.2216/16-14.1

YIC-IR > 海岸带生物学与生物资源利用重点实验室 > 海岸带生物学与生物资源保护实验室

	Isolation and characterization of astaxanthin-hyperproducing mutants of Haematococcus pluvialis (Chlorophyceae) produced by dielectric barrier discharge plasma
	Liu, Jinghua ; Chen, Jun ; Chen, Zhu ; Qin, Song; Huang, Qing ; Huang, Q (reprint author), Chinese Acad Sci, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Key Lab Ion Beam Bioengn, Hefei 230031, Anhui, Peoples R China.; Huang, Q (reprint author), Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China.; Qin, S (reprint author), Chinese Acad Sci, YanTai Inst Coastal Zone Res, Yantai 264003, Peoples R China. sqin@yic.ac.cn ; huangq@ipp.ac.cn
发表期刊	PHYCOLOGIA
ISSN	0031-8884
	2016
卷号	55 期号:6 页码:650-658
关键词	Astaxanthin Carotenogenesis Dielectric Barrier Discharge Haematococcus Pluvialis Lycopene Cyclase Mutagenesis Nonthermal Plasma Photosynthetic Activity Quantitative Real-time Pcr Random Amplification Of Polymorphic Dna
DOI	10.2216/16-14.1
产权排序	[Liu, Jinghua; Chen, Zhu; Huang, Qing] Chinese Acad Sci, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Key Lab Ion Beam Bioengn, Hefei 230031, Anhui, Peoples R China; [Liu, Jinghua; Huang, Qing] Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China; [Chen, Jun; Qin, Song] Chinese Acad Sci, YanTai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Chen, Jun] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
作者部门	海岸带生物学与生物资源利用所重点实验室
英文摘要	Haematococcus pluvialis has potential commercial application for its ability to accumulate astaxanthin under stress conditions. To improve astaxanthin productivity, nonthermal plasma can be used to produce mutant algal strains with enhanced production of astaxanthin. In this work, atmospheric pressure argon dielectric barrier discharge (DBD) was used for mutation induction. Several new algal strains with increased growth rate and higher astaxanthin accumulation were obtained. For analysis of the DBD-treated algal mutants, genetic variation was examined by random amplification of polymorphic DNA, and the pigment pattern in response to inhibitors (diphenylamine and nicotine) of carotenoid biosynthetic pathway was characterized by high-performance liquid chromatography. Furthermore, photosynthetic activity and gene expression were also examined by pulse amplitude-modulated fluorometry and quantitative real-time polymerase chain reaction, respectively. Our results confirmed that the enhanced astaxanthin accumulation in the mutants was associated with increased expression levels of lycopene cyclase, which is involved in the conversion of lycopene to beta-carotene. As such, this work demonstrates a successful example for the effective application of DBD in the mutation breeding of astaxanthin-hyperproducing algal strains.
文章类型	Article
资助机构	National Natural Science Foundation of China(11475217) ; Strategic Priority Research Program of the Chinese Academy of Sciences(XDA08040107)
收录类别	SCI
语种	英语
关键词[WOS]	GREEN-ALGA ; CAROTENOID ACCUMULATION ; GENES ; MUTAGENESIS ; EXPRESSION ; FLUORESCENCE ; MUTATION ; GROWTH ; ARTP
研究领域[WOS]	Plant Sciences ; Marine & Freshwater Biology
WOS记录号	WOS:000390263000004
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.yic.ac.cn/handle/133337/19252
专题	海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者	Huang, Q (reprint author), Chinese Acad Sci, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Key Lab Ion Beam Bioengn, Hefei 230031, Anhui, Peoples R China.; Huang, Q (reprint author), Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China.; Qin, S (reprint author), Chinese Acad Sci, YanTai Inst Coastal Zone Res, Yantai 264003, Peoples R China. sqin@yic.ac.cn; huangq@ipp.ac.cn
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Tech Biol & Agr Engn, Key Lab Ion Beam Bioengn, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 3.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 4.Chinese Acad Sci, YanTai Inst Coastal Zone Res, Yantai 264003, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Jinghua,Chen, Jun,Chen, Zhu,et al. Isolation and characterization of astaxanthin-hyperproducing mutants of Haematococcus pluvialis (Chlorophyceae) produced by dielectric barrier discharge plasma[J]. PHYCOLOGIA,2016,55(6):650-658.
APA	Liu, Jinghua.,Chen, Jun.,Chen, Zhu.,Qin, Song.,Huang, Qing.,...&huangq@ipp.ac.cn.(2016).Isolation and characterization of astaxanthin-hyperproducing mutants of Haematococcus pluvialis (Chlorophyceae) produced by dielectric barrier discharge plasma.PHYCOLOGIA,55(6),650-658.
MLA	Liu, Jinghua,et al."Isolation and characterization of astaxanthin-hyperproducing mutants of Haematococcus pluvialis (Chlorophyceae) produced by dielectric barrier discharge plasma".PHYCOLOGIA 55.6(2016):650-658.