Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads
Zhao, Guangtao1,2,3; Ding, Jiawang1,2; Yu, Han1,2,3; Yin, Tanji1,2; Qin, Wei1,2; Yin, TJ; Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China.; Qin, W (reprint author), YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China. gtzhao@yic.ac.cn; jwding@yic.ac.cn; hyu@yic.ac.cn; tjyin@yic.ac.cn; wqin@yic.ac.cn
2016-12-01
发表期刊SENSORS
ISSN1424-8220
卷号16期号:12页码:.
产权排序[Zhao, Guangtao; Ding, Jiawang; Yu, Han; Yin, Tanji; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China; [Zhao, Guangtao; Ding, Jiawang; Yu, Han; Yin, Tanji; Qin, Wei] YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China; [Zhao, Guangtao; Yu, Han] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
摘要A potentiometric aptasensing assay that couples the DNA nanostructure-modified magnetic beads with a solid-contact polycation-sensitive membrane electrode for the detection of Vibrio alginolyticus is herein described. The DNA nanostructure-modified magnetic beads are used for amplification of the potential response and elimination of the interfering effect from a complex sample matrix. The solid-contact polycation-sensitive membrane electrode using protamine as an indicator is employed to chronopotentiometrically detect the change in the charge or DNA concentration on the magnetic beads, which is induced by the interaction between Vibrio alginolyticus and the aptamer on the DNA nanostructures. The present potentiometric aptasensing method shows a linear range of 10-100 CFU mL(-1) with a detection limit of 10 CFU mL(-1), and a good specificity for the detection of Vibrio alginolyticus. This proposed strategy can be used for the detection of other microorganisms by changing the aptamers in the DNA nanostructures.
关键词Potentiometric Aptasensing Vibrio Alginolyticus Dna Nanostructures Magnetic Beads Protamine
作者部门中科院海岸带环境过程与生态修复重点实验室
学科领域Chemistry ; Electrochemistry ; Instruments & Instrumentation
DOI10.3390/s16122052
项目资助者Chinese Academy of Sciences(XDA11020702) ; National Natural Science Foundation of China(41176081 ; Taishan Scholar Program of Shandong Province ; 21475148 ; 21575158)
收录类别SCI
关键词[WOS]IONIC LIQUID ; CARBON NANOTUBES ; ELECTRODE ; APTAMERS ; ASSAYS ; RECOGNITION ; PERFORMANCE ; INFECTIONS ; BIOSENSOR ; PROTAMINE
文章类型Article
语种英语
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
WOS记录号WOS:000391303000072
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cn/handle/133337/19248
专题中科院海岸带环境过程与生态修复重点实验室
通讯作者Yin, TJ; Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China.; Qin, W (reprint author), YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China. gtzhao@yic.ac.cn; jwding@yic.ac.cn; hyu@yic.ac.cn; tjyin@yic.ac.cn; wqin@yic.ac.cn
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China
2.YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
第一作者单位中国科学院烟台海岸带研究所
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Zhao, Guangtao,Ding, Jiawang,Yu, Han,et al. Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads[J]. SENSORS,2016,16(12):..
APA Zhao, Guangtao.,Ding, Jiawang.,Yu, Han.,Yin, Tanji.,Qin, Wei.,...&wqin@yic.ac.cn.(2016).Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads.SENSORS,16(12),..
MLA Zhao, Guangtao,et al."Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads".SENSORS 16.12(2016):..
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