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题名:
Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads
作者: Zhao, Guangtao1,2,3; Ding, JiaWang(丁家旺)1,2; Yu, Han1,2,3; Yin, TanJi(尹坦姬)1,2; Wei Qin(秦伟)1,2
刊名: SENSORS
ISSN号: 1424-8220
出版日期: 2016-12-01
卷号: 16, 期号:12, 页码:.
关键词: potentiometric aptasensing ; Vibrio alginolyticus ; DNA nanostructures ; magnetic beads ; protamine
学科分类: Chemistry ; Electrochemistry ; Instruments & Instrumentation
DOI: 10.3390/s16122052
产权排序: [Zhao, Guangtao; Ding, Jiawang; Yu, Han; Yin, Tanji; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China; [Zhao, Guangtao; Ding, Jiawang; Yu, Han; Yin, Tanji; Qin, Wei] YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China; [Zhao, Guangtao; Yu, Han] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者: Yin, TJ ; Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China. ; Yin, TJ ; Qin, W (reprint author), YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China. gtzhao@yic.ac.cn ; jwding@yic.ac.cn ; hyu@yic.ac.cn ; tjyin@yic.ac.cn ; wqin@yic.ac.cn
作者部门: 中科院海岸带环境过程与生态修复重点实验室
英文摘要: A potentiometric aptasensing assay that couples the DNA nanostructure-modified magnetic beads with a solid-contact polycation-sensitive membrane electrode for the detection of Vibrio alginolyticus is herein described. The DNA nanostructure-modified magnetic beads are used for amplification of the potential response and elimination of the interfering effect from a complex sample matrix. The solid-contact polycation-sensitive membrane electrode using protamine as an indicator is employed to chronopotentiometrically detect the change in the charge or DNA concentration on the magnetic beads, which is induced by the interaction between Vibrio alginolyticus and the aptamer on the DNA nanostructures. The present potentiometric aptasensing method shows a linear range of 10-100 CFU mL(-1) with a detection limit of 10 CFU mL(-1), and a good specificity for the detection of Vibrio alginolyticus. This proposed strategy can be used for the detection of other microorganisms by changing the aptamers in the DNA nanostructures.
研究领域[WOS]: Chemistry ; Electrochemistry ; Instruments & Instrumentation
项目资助者: Chinese Academy of Sciences(XDA11020702) ; National Natural Science Foundation of China(41176081 ; Taishan Scholar Program of Shandong Province ; 21475148 ; 21575158)
关键词[WOS]: IONIC LIQUID ; CARBON NANOTUBES ; ELECTRODE ; APTAMERS ; ASSAYS ; RECOGNITION ; PERFORMANCE ; INFECTIONS ; BIOSENSOR ; PROTAMINE
文章类型: Article
收录类别: SCI
语种: 英语
WOS记录号: WOS:000391303000072
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.yic.ac.cn/handle/133337/19248
Appears in Collections:中科院海岸带环境过程与生态修复重点实验室_期刊论文

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作者单位: 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China
2.YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

Recommended Citation:
Zhao, Guangtao,Ding, Jiawang,Yu, Han,et al. Potentiometric Aptasensing of Vibrio alginolyticus Based on DNA Nanostructure-Modified Magnetic Beads[J]. SENSORS,2016,16(12):..
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